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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Human |
表达系统 |
E. coli |
氨基酸序列 |
Human MMP-14, amino acids Tyr89-Gly265 |
分子量 |
20 kDa |
分类 |
Recombinant |
类型 |
Protein |
活性 |
Specific Activity > 150 mU/mg; with 1 U defined as the activity that hydrolyzes 1 µmol peptide within 1 minute in an enzyme assay. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.2 mg/mL |
纯化类型 |
purified |
保存液 |
TBS, pH 7.5, with 5mM CaCl2 |
内含物 |
no preservative |
保存条件 |
-80° C, Avoid Freeze/Thaw Cycles |
RP-77531 is a purified recombinant MMP-14 (catalytic domain) protein, expressed in E.coli. MT1-MMP catalytic domain is produced by activation of a recombinant soluble proform of MT1-MMP purified from E. coli periplasm. The catalytic domain is inhibited by tissue inhibitors of matrix metalloproteinases 2 and 3 (TIMP-2 and TIMP-3) and by chelators of divalent cations like EDTA or o-phenanthroline. This product can be used to study the activation of TIMP-2 and the degradation of proteins of the extracellular matrix. The enzyme allows screening of TIMP inhibitors and characterization of inhibitor action.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: EC 3.4.24.80; matrix metallo protease; matrix metallopeptidase 14 (membrane-inserted); Matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP; MMP-14; MMP-X MT-MMP; MMP-X1; MMPs; MT-MMP 1; MT-MMP MT1-MMP; MT1-MMP
基因别名: MMP-14; MMP-X1; MMP14; MT-MMP; MT-MMP 1; MT1-MMP; MT1MMP; MTMMP1; WNCHRS
UniProt ID: (Human) P50281
Entrez Gene ID: (Human) 4323
分子生物学功能:
metalloprotease
