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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
其他PubMed (Misc) |
- | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Human |
已发表种属 |
Not Applicable |
表达系统 |
E. coli |
氨基酸序列 |
Human MMP-9, amino acids 113-450 with an N-terminal His-tag |
标签 |
His-tag |
分子量 |
42.03 kDa |
分类 |
Recombinant |
类型 |
Protein |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.265 mg/mL |
纯化类型 |
purified |
保存液 |
20mM tris HCl, pH 8.0, with 50% glycerol |
内含物 |
no preservative |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
This product contains purified recombinant human MMP-9 protein with an N-terminal hexahistidine tag. The recombinant protein is a fragment corresponding to the catalytic domain of the protein (residues 113-450).
This product may be used as a positive control in many immunological assays.
MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase-9; Matrix metalloproteinase9; MMP; MMP-9; MMPs; type V collagenase
基因别名: CLG4B; GELB; MANDP2; MMP-9; MMP9
UniProt ID: (Human) P14780
Entrez Gene ID: (Human) 4318
分子生物学功能:
metalloprotease
