应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
产品规格 | |
---|---|
种属 |
Human |
表达系统 |
E. coli |
分子量 |
17.5 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>95% by SDS-PAGE |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 = 0.02 - 0.05 ng/mL; determined by the dose-dependent cytotoxcity on L929 cells in the presence of actinomycin D. |
偶联物 |
Unconjugated |
形式 |
Liquid |
纯化类型 |
sequential chromatography |
保存液 |
40mM tris, pH 8 |
内含物 |
no preservative |
保存条件 |
-20°C |
Carrier-Free
Storage: Store the liquid human TNF-alpha at ≤-20°C. Upon thawing, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: APC1 protein; C-domain 1; C-domain 2; Cachectin; DADB-70P7.1; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha
基因别名: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F
UniProt ID: (Human) P01375
Entrez Gene ID: (Human) 7124