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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
中和实验 (Neu) |
- | 查看1篇已发表文章 1篇已发表文章 |
对照试剂 (Ctrl) |
Assay-dependent | - |
抑制试剂 (IA) |
Cell studies, 8.0-20 µM | - |
其他PubMed (Misc) |
- | 查看3篇已发表文章 3篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Human |
已发表种属 |
Human |
表达系统 |
E. coli |
分子量 |
46.7 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>80% |
活性 |
For furin inhibition assays, cells should be treated with 8.0 - 20 µM human alpha-1 PDX recombinant protein for 1 hour. For in vitro assays, 0.1 - 2.0 µM human alpha-1 PDX recombinant protein should be used. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
浓度 |
2.5 mg/mL |
纯化类型 |
purified |
保存液 |
PBS, pH 7.2 |
内含物 |
no preservative |
保存条件 |
4° C, do not freeze |
RP-070 was obtained from E. coli transformed with the alpha1-PDX gene. RP-070 can be used in furin inhibition assays. For furin inhibition assays, cells should be treated with 8.0-20 µM alpha1-PDX for 1 hour (see above references). For in vitro assays, 0.1-2.0 µM alpha1-PDX should be used. Purity: >80% (most lots are 80-95%) NOTE: Freezing this product may result in damage of the protein. NOTE: Once rehydrated, this protein retains optimal reactivity for 2-4 days at 4 degrees C. Longer storage time can be achieved by storing in a serum rich medium. RP-070 can be used for protein biochemical, intracellular trafficking, and protein kinetic assays.
Molecular weight is approximately 46.7 kDa.
Alpha1-PDX is a potent and selective inhibitor of furin (Ki = 0.6 nM). Alpha1-PDX was engineered by the construction of a variant of alpha1-antitrypsin that contains a minimal furin consensus sequence, RXXR. Cellular expression of alpha1-PDX effectively blocks the furin-dependent processing of protein precursors. In addition, extracellular application of alpha1-PDX can also block the processing of furin substrates. Alpha1-PDX has been shown to inhibit the processing of biologically significant proteins including many viral envelope glycoproteins, bacterial toxins, receptors and growth factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: alpha1 PDX; Pyruvate Dehydrogenase Complex Component-X
