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IkB zeta Antibody (740065T) in ICC/IF
Immunofluorescent analysis of IκB Zeta was performed using 70% confluent RAW 264.7 cells with or without LPS treatment (1 µg/mL, 6 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with IκB Zeta Recombinant Rat Monoclonal Antibody (LK2NAP) (Product # 740065T, 1:200). Panels a) and e) shows representative images of cells that were stained for detection and localization of IκB Zeta. Panels b) ... View More
产品信息
740065T
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rat
/ IgG2a
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
LK2NAP
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
I-kappa-B-zeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ30225; FLJ34463; I-kappa-B-zeta; Ikappa B-zeta variant 3; IkappaB-zeta; ikappaBzeta; IkB-zeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; MAIL; Molecule possessing ankyrin repeats induced by lipopolysaccharide; molecule possessing ankyrin-repeats induced by lipopolysaccharide; NF-kappa-B inhibitor zeta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta; OTTHUMP00000214340
基因别名: AA408868; IKBZ; INAP; MAIL; NFKBIZ; RGD1310834
UniProt ID: (Human) Q9BYH8, (Mouse) Q9EST8
Entrez Gene ID: (Human) 64332, (Rat) 304005, (Mouse) 80859
