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IkB zeta Antibody (740065TP488) in Flow
Flow cytometry analysis of IkB zeta was performed using RAW 264.7cells and RAW 264.7 cells treated with LPS (1 µg/mL) for 6 hours. Cells were fixed with Foxp3 / Transcription Factor Staining Buffer Set (Product # 00-5523-00), permeabilized and stained intracellularly with 0.25 µg of IkB zeta Recombinant Rat Monoclonal Antibody (LK2NAP), Alexa Fluor™ Plus 488 (Product # 740065TP488). An increased expression of IkB zeta was observed in treated cells (pink histogram) compared to untreated cells (blue histogram). Viable cells were used for ana... View More
产品信息
740065TP488
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
LK2NAP
偶联物
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.8
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This LK2NAP antibody has been tested by western blot and flow cytometric analysis of RAW 264.7 cells treated with LPS. This may be used for western blot at 5 µg/mL and for flow cytometry at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
I-kappa-B-zeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ30225; FLJ34463; I-kappa-B-zeta; IkappaB-zeta; ikappaBzeta; IkB-zeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; MAIL; Molecule possessing ankyrin repeats induced by lipopolysaccharide; molecule possessing ankyrin-repeats induced by lipopolysaccharide; NF-kappa-B inhibitor zeta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta; OTTHUMP00000214340
基因别名: AA408868; INAP; Mail; Nfkbiz
UniProt ID: (Mouse) Q9EST8
Entrez Gene ID: (Mouse) 80859
