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Invitrogen
MAP2 Monoclonal Antibody (AP20)
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图: 1 / 9
MAP2 Antibody (MA5-12823) in ICC/IF
Immunofluorescence analysis of neurons using anti-MAP2 antibody (Product # MA5-12823). GABAergic precursor cells derived from H9 embryonic stem cells were differentiated for 7 days and stained with antibodies against MAP2 (Product # MA5-12823 at 1:200) followed by Alexa Fluor 488 goat anti-rabbit (Product # A-21206, green). Nuclear DNA was stained with DAPI (blue) in the merged image (right panel).
产品信息
MA5-12823
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human
已发表种属
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
AP20
抗原
Bovine brain microtubule associated protein 2
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
tissue culture supernatant
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is specific to MAP2a and MAP2b, and will not cross-react with MAP1, MAP5, tubulin or tau. This clone reacts with dendrites and cell bodies of neurons but not with the neuronal processes. It does not react with the low MW form (70 kDa, MAP2c). The epitope has been mapped to aa 997-1332.
The molecular weight of the doublet is ~280 kDa.
For IHC staining, formalin-fixed tissues must be boiled in a 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Recommened positive controls include human glioblastoma T98G cells or brain tissue.
靶标信息
MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AC091465.1; DKFZp686I2148; MAP 2; MAP-2; map-2a; map2ab; microtubule associated protein 2C; Microtubule-associated protein 2; microtubule-associated protein-2; OTTHUMP00000163916; OTTHUMP00000208143
基因别名: MAP2; MAP2A; MAP2B; MAP2C
UniProt ID: (Human) P11137
Entrez Gene ID: (Human) 4133, (Bovine) 281294
