Invitrogen

METTL1 Polyclonal Antibody

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METTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human METTL1 in human bladder carcinoma with METTL1 Polyclonal Antibody (Product # PA5-80810, 1:5,000 dilution, formalin-fixed paraffin embedded sections). Left panel: tissue incubated with primary antibody. Right panel: tissue incubated with mixture of primary antibody and antigen (recombinant protein).

METTL1 Antibody in Western Blot (WB) — Western Blot using METTL1 Polyclonal Antibody (Product # PA5-80810) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa. (We are unsure of the identity of these extra bands).

METTL1 Antibody in Immunoprecipitation (IP) — METTL1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate 0.5 µL with METTL1 Polyclonal Antibody (Product # PA5-80810) and 15 µL of 50 % Protein G agarose. Primary antibody: METTL1 Polyclonal Antibody, at 1:1,000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.

产品信息

PA5-80810

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:2,000-1:10,000

酶联免疫吸附实验 (ELISA)

1:25000-1:50000

免疫沉淀 (IP)

0.2-1 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human METTL1 protein (Asp32-Gln265)

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

RRID

AB_2788084

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human METTL1.

靶标信息

This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: D1075-like gene product; Methyltransferase-like protein 1; METTL1; miRNA (guanine-N(7)-)-methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine(46)-N(7))-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase

基因别名: C12orf1; METTL1; TRM8; TRMT8; YDL201w

UniProt ID: (Human) Q9UBP6

Entrez Gene ID: (Human) 4234

分子生物学功能: RNA processing factor

功能

tRNA modification tRNA methylation RNA (guanine-N7)-methylation

参与通路

tRNA binding protein binding tRNA (guanine-N7-)-methyltransferase activity