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Invitrogen
MIB1 Polyclonal Antibody
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MIB1 Antibody (PA5-97126) in WB
Western blot analysis of MIB1 in extracts of various cell lines. Samples were incubated with MIB1 Polyclonal antibody (Product # PA5-97126) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-97126
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 560-860 of human MIB1/DIP1 (NP_0658251)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.31 mg/mL
规格
131 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: DSEGDTPLHD AISKKRDDIL AVLLEAGADV TITNNNGFNA LHHAALRGNP SAMRVLLSKL PRPWIVDEKK DDGYTALHLA ALNNHVEVAE LLVHQGNANL DIQNVNQQTA LHLAVERQHT QIVRLLVRAG AKLDIQDKDG DTPLHEALRH HTLSQLRQLQ DMQDVGKVDA AWEPSKNTLI MGLGTQGAEK KSAASIACFL AANGADLSIR NKKGQSPLDL CPDPNLCKAL AKCHKEKVSG QVGSRSPSMI SNDSETLEEC MVCSDMKRDT LFGPCGHIAT CSLCSPRVKK CLICKEQVQS R; Positive Samples: U-87MG, A-549, HepG2, Mouse stomach, Mouse lung, Mouse kidney, Rat testis; Cellular Location: Cell membrane, Cytoplasm, centriolar satellite, centrosome, cytoskeleton, microtubule organizing center
靶标信息
MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DAPK-interacting protein 1; DAPK-interacting protein-1; DIP-1; E3 ubiquitin-protein ligase MIB1; Mind bomb homolog 1; mind bomb-1; mindbomb homolog 1; RING-type E3 ubiquitin transferase MIB1; skeletrophin
基因别名: DIP-1; Dip1; E430019M12Rik; Kiaa1323; Mib; Mib1; mKIAA1323
UniProt ID: (Mouse) Q80SY4
Entrez Gene ID: (Mouse) 225164, (Rat) 307594
ubiquitin-protein transferase activity
protein binding
zinc ion binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
molecular_function
ubiquitin-protein ligase
protein modifying enzyme
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