Rockland

Mcl-1 Polyclonal Antibody

Name: Mcl-1 Polyclonal Antibody
Brand: Rockland
SKU: 600-401-394

查看其他37个MCL-1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

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Mcl-1 Antibody (600-401-394) in WB

Western blot analysis using Rocklands anti-Mcl-1 antibody to detect Mcl-1 in MEF cell lysates (20 µg per lane). Anti-Mcl-1 was used at a dilution of 1:10,000 followed by reaction with HRP Anti-Rabbit IgG [H&L] MX10 (GOAT) used at a 1:2,000 dilution. Western Lightning Chemiluminescence Reagent Plus from Perkin Elmer was used for detection. The exposure time was exactly 30 seconds. This antibody detects 35 kDa mouse Mcl-1. Communicated with permission by J. Opferman and S. Korsmeyer. See Opferman et al (2003) for additional details.

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产品信息

600-401-394

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10,000

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.2 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

靶标信息

MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and bcl-2 share significant sequence homology. Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S. In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.

仅用于科研。不用于诊断过程。未经明确授权不得转售。