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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
功能检测 (FN) |
Assay-Dependent | - |
对照试剂 (Ctrl) |
Assay-Dependent | - |
其他PubMed (Misc) |
- | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Mouse |
已发表种属 |
Not Applicable |
表达系统 |
E. coli |
氨基酸序列 |
Mouse CD40L, amino acids Met112-Leu250 (Accession # P27548) |
分子量 |
16 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>98% by SDS-PAGE and HPLC |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 = 0.1 µg/mL; as measured by the induction of TNF-alpha and MIP-1alpha production by mouse splenocytes. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
Affinity chromatography |
保存液 |
0.01M sodium phosphate, pH 7.5, with 0.1M arginine |
内含物 |
no preservative |
保存条件 |
-20°C |
Description: CD40 ligand (CD40L, also known as CD154, TRAP or gp39) is a membrane glycoprotein expressed on activated CD4+ T-cells, NK cells, mast cells, basophils and eosinophils. The CD40-CD40L interaction stimulates B cell immune responses including cell surface antigen expression, cell cycle activation, Ig isotype switching, Ig secretion and memory generation. The CD40-CD40L interaction also plays important roles in monocyte and dendritic cell activation, T-cell co-stimulation and cytokine production. It has been reported that the CD40-CD40L interaction is involved in the pathogenesis of amyloid pathology in Alzheimer disease. E. coli-produced recombinant mouse CD40L is a non-glycosylated polypeptide of 149 amino acids comprising the soluble extracellular domain of CD40L.
Applications Reported: Recombinant mouse CD154 (CD40 Ligand) is biologically active and can promote TNFa and MIP-1a production by mouse splenocytes in culture.
Applications Tested: The ED50 of this protein, as measured by induction of TNFa and MIP-1a production by mouse splenocytes, is less than 0.1 µg/mL.
Source: E. coli (amino acids Met112-Leu250; accession # P27548).
Bioactivity: The ED50 of this protein, as measured by induction of TNFa and MIP-1a production by mouse splenocytes, is less than 0.1 µg/mL.
Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC.
Molecular Weight: 16 kDa.
Storage and handling: Use in sterile environment. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier protein (e.g., 0.1% BSA) at less than or equal to -20°C.
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: CD154; CD40 ligand; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; sCD40L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5
基因别名: CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP
UniProt ID: (Mouse) P27548
Entrez Gene ID: (Mouse) 21947
