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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Mouse |
表达系统 |
E. coli |
氨基酸序列 |
KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK |
分子量 |
7.9 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>98% by SDS-PAGE |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 = 50 - 100 ng/mL; determined by the dose-dependent chemotaxis of human PBMCs. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
sequential chromatography |
内含物 |
no preservative |
保存条件 |
-20°C |
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyophilized protein should be reconstituted in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in tissue culture grade buffered solutions with heat inactivated fetal calf serum or low endotoxin BSA.
Storage: Lyophilized human SDF-1 alpha should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted human SDF-1 alpha at < -20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; hIRH; hSDF-1; PBSF; Pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SDF-1; SDF-1a; SDF-1b; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; Thymic lymphoma cell-stimulating factor; TLSF; TLSF-a; TLSF-b; Tlsfa; Tlsfb; TPAR1
基因别名: Cxcl12; Pbsf; Scyb12; Sdf1; Tlsf; Tpar1
UniProt ID: (Mouse) P40224
Entrez Gene ID: (Mouse) 20315
