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Gibco
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | 查看5篇已发表文章 5篇已发表文章 |
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Mouse |
已发表种属 |
Human, Mouse |
表达系统 |
E. coli |
氨基酸序列 |
NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR |
分子量 |
6 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>95% by SDS-PAGE |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 < 5 ng/mL; determined by the dose-dependent proliferation of BALB/c 3T3 cells. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
sequential chromatography |
内含物 |
no preservative |
保存条件 |
-20°C |
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile PBS to a concentration of 0.1-1.0 mg/mL. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in aqueous buffers supplemented with carrier protein, such as 0.1-1.0% BSA.
Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF)
基因别名: AI790464; Egf
UniProt ID: (Mouse) Q6P9J2
Entrez Gene ID: (Mouse) 13645
分子生物学功能:
transmembrane signal receptor
