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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
其他PubMed (Misc) |
- | 查看2篇已发表文章 2篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Mouse |
已发表种属 |
Not Applicable |
表达系统 |
E. coli |
分子量 |
19.3 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>95% |
内毒素浓度 |
<1 EU/µg |
活性 |
EC50 ~ 5 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394] with the exception that EMCV rather than VSV was used as the challenge. The activity was determined relative to a lab standard of Mu IFN-alpha-A which was calibrated to the NIH mouse IFN-alpha- standard (Ga02-901-511). |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
Lot-specific |
纯化类型 |
Ion-exchange chromatography, Size-exclusion chromatography |
保存液 |
PBS with 0.1% BSA |
内含物 |
no preservative |
保存条件 |
-80° C, Avoid Freeze/Thaw Cycles |
Store at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2 x 10^5 units/mL is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: ifa; IFN alpha 1/13; IFN alpha 1b; IFN α; IFN-alpha-1; IFN-alpha-2; IFNAalphaIFN-alpha-1/13; IFNalpha; IFNα; Interferon alpha 13 (IFNA13); interferon alpha 15; interferon alpha family gene 1; interferon alpha family, gene 2; Interferon alpha leukocyte (IFL); Interferon alpha-1; Interferon alpha-1/13 (IFN-alpha-1/13); interferon alpha-2; Interferon alpha-D (IFN alphaD); Interferon alpha1 (IFNA1); MGC138207; MGC138505; MGC138507; novel interferon alpha (Ifna) family member
基因别名: Gm12597; Ifa1; Ifa2; Ifna1; Ifnaa; MuIFN-alpha-A; OTTMUSG00000007655
UniProt ID: (Mouse) P01572
Entrez Gene ID: (Mouse) 15962, (Mouse) 242517, (Mouse) 15965
分子生物学功能:
interferon superfamily
