应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
产品规格 | |
---|---|
种属 |
Mouse |
表达系统 |
E. coli |
分子量 |
19.3 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
≥95% |
活性 |
Specific Activity = 7.25x10e7 units/mg; The biological activity is determined by titration with the use of the cytopathic effect inhibition assay (Rubinstein et al., 1981). In this antiviral assay for interferon, about 1 unit/mL of interferon is the quantity necessary to inhibit the cytopathic effect by 50% using L929 cells challenged with encephalomyocarditis virus. The units are determined with respect to the international reference standard for mouse interferon alpha (mouse-IFN-α) provided by the National Institutes of Health (Pestka, 1986). |
偶联物 |
Unconjugated |
形式 |
Liquid |
纯化类型 |
purified |
保存液 |
PBS with 0.1% BSA |
内含物 |
no preservative |
保存条件 |
-80°C |
Dilution Recommendation: Polypropylene tubes and pipet tips should be used when diluting this interferon. Further dilutions should be made in buffered solution containing a carrier protein, such as BSA. The optimal concentration should be determined for each specific application.
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: IFN-a; IFN-alpha 1b; IFN-alpha-2; IFNA 2; interferon alpha 1b; interferon alpha family, gene 2; interferon alpha-2; interferon alpha-D; Interferon-alpha II; leIF D; RP11-354P17.2
基因别名: Ifa2
Entrez Gene ID: (Mouse) 15965
分子生物学功能:
interferon superfamily