应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
产品规格 | |
---|---|
种属 |
Mouse |
表达系统 |
E. coli |
氨基酸序列 |
CYCHGTVIES LESLNNYFNS SGIDVEEKSL FLDIWRNWQK DGDMKILQSQ IISFYLRLFE VLKDNQAISN NISVIESHLI TTFFSNSKAK KDAFMSIAKF EVNNPQVQRQ AFNELIRVVH QLLPESSLRK RKRSR |
分子量 |
15 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>95% by SDS-PAGE |
内毒素浓度 |
<0.1 ng/mL |
活性 |
ED50 < 0.6 ng/mL; determined by a dose-dependent cytostasis assay of mouse WeHi279 cells. |
偶联物 |
Unconjugated |
形式 |
Liquid |
纯化类型 |
sequential chromatography |
保存液 |
tris, pH 7.4 |
内含物 |
no preservative |
保存条件 |
-80°C |
•Purity: >95% by SDS-PAGE
•Endotoxin Concentration: <0.1 ng/mL
•Biological Activity: ED50 <0.600 ng/mL
•Molecular Weight: 15 kDa
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g
基因别名: Ifg; IFN-g; Ifng
UniProt ID: (Mouse) P01580
Entrez Gene ID: (Mouse) 15978
分子生物学功能:
cytokine
interferon superfamily
intercellular signal molecule