应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
其他PubMed (Misc) |
- | 查看2篇已发表文章 2篇已发表文章 |
产品规格 | |
---|---|
种属 |
Mouse |
已发表种属 |
Not Applicable |
表达系统 |
Yeast |
氨基酸序列 |
Mouse IL-1 beta, amino acids 118-269 (Accession # P10749) |
分子量 |
17.4 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>95% by SDS-PAGE |
活性 |
Determined by the proliferation of mouse D10S cells. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
purified |
保存液 |
PBS |
内含物 |
no preservative |
保存条件 |
4° C |
Mature mouse IL-1b was expressed in Pichia pastoris, X-33. GenBank Accession # P10749, amino acids 118 - 269. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: VPIRQLHYRL. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant mouse IL-1b was determined by measuring the proliferation of mouse D10S cells.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta
基因别名: Il-1b; IL-1beta; Il1b
UniProt ID: (Mouse) P10749
Entrez Gene ID: (Mouse) 16176
分子生物学功能:
interleukin superfamily