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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
酶联免疫吸附实验 (ELISA) |
Assay-Dependent | - |
功能检测 (FN) |
Assay-Dependent | - |
对照试剂 (Ctrl) |
Assay-Dependent | - |
其他PubMed (Misc) |
- | 查看6篇已发表文章 6篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Mouse |
已发表种属 |
Not Applicable |
表达系统 |
E. coli |
氨基酸序列 |
Mature mouse IL-17A, amino acids Thr22-Ala158 (Accession # NM_010552) |
分子量 |
15.4 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>98% by SDS-PAGE |
内毒素浓度 |
<0.01 ng/µg |
活性 |
ED50 = 3 ng/mL; determined by the dose-dependent induction of IL-6 production by NIH/3T3 cells. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.1 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
0.1M glycine, 1% BSA |
内含物 |
no preservative |
保存条件 |
-80°C |
Description: Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A binds to a receptor that binds also to HVS13 (viral IL-17A) and to CTLA-8.
Applications Reported: Recombinant mouse IL-17A is biologically active and can promote IL-6 production in vitro. Recombinant mouse IL-17A is useful as an ELISA standard.
Applications Tested: The recombinant mouse IL-17A has been tested as the standard in a mouse IL-17 sandwich ELISA and in bioassay for induction of IL-6 production by NIH/3T3 cells. The ED50 is 3.0 ng/mL, corresponding to a specific activity of 3.3 x 10E5 Units/mg.
Source: E. coli expressed amino acids Thr 22-Ala 158 of mature mouse IL-17A accession # NM_010552.
Bioactivity: Measured by induction of IL-6 production by NIH/3T3 cells. The ED50 is 3.0 ng/mL, corresponding to a specific activity of 3.3 x 10e5 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The protein does not contain an N-terminal methionine. The polypeptide has a predicted molecular mass of 15,377. The DTT reduced protein migrates as a 15 kDa polypeptide. The nonreduced cystine linked protein migrates as a 30 kDa protein on non-reducing SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; il 17; IL-17; ILN; Interleukin; interleukin 17; Interleukin-17A; Interleukin17A; R-IL-17-A
基因别名: Ctla-8; Ctla8; IL-17; IL-17A; Il17; Il17a
UniProt ID: (Mouse) Q62386
Entrez Gene ID: (Mouse) 16171
