应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
产品规格 | |
---|---|
种属 |
Mouse |
表达系统 |
E. coli |
氨基酸序列 |
Mouse IL-18, amino acids 36-192 of the mature sequence |
分子量 |
18 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>90% by SDS-PAGE |
内毒素浓度 |
<0.1 ng/µg |
活性 |
Determined by the dose-dependent induction of IFN-gamma production in mouse IL-18 receptor transfected KG-1 cells. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
sequential chromatography |
保存液 |
PBS with 0.1% BSA, 1% sucrose |
内含物 |
no preservative |
保存条件 |
-20°C |
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the contents of the vial with 250 µL ice cold, sterile, distilled water on ice.
Storage: Store this lyophilized preparation at -20°C, preferably desiccated. Upon reconstitution, the protein may be stored at 2°C to 8°C for up to one week. For long term storage of the reconstituted protein, apportion into working aliquots and store at -80°C. Avoid repeated freeze/thaw cycles.
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: IFN-gamma-inducing factor; il 18; IL-1 gamma; IL-18; ILN; Interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320
基因别名: Igif; Il-18; Il18
UniProt ID: (Mouse) P70380
Entrez Gene ID: (Mouse) 16173
分子生物学功能:
interleukin superfamily