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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
其他PubMed (Misc) |
- | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Mouse |
已发表种属 |
Not Applicable |
表达系统 |
E. coli |
分子量 |
15.1 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>96% by SDS-PAGE and HPLC analysis |
活性 |
ED50 < 0.1 ng/mL; determined by dose-dependent proliferation of mouse FDC-P1 cells. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
purified |
内含物 |
no preservative |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
RMIL310 contains 134 amino acid residues.
Purity: >96% pure by SDS-PAGE and HPLC analysis.
Activity: One unit of activity is <0.1 ng/mL, as determined by proliferation assay of mouse IL-3-dependent FDC-P1 cells, corresponding to a specific activity of >1x10^7 units/mg.
Use: Interleukin-3 exerts activity in the range of 0.1 to 10 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 µg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: BP/BPA; Colony stimulating factor multiple; Eo-CSF; Hematopoietic growth factor; IL-3; ILN; Interleukin; Interleukin 3 (colony stimulating factor; Interleukin-3; Interleukin3; Mast cell growth factor; MCGF; MGC79398; MGC79399; Multi CSF; Multilineage colony stimulating factor; multiple); Multipotential colony-stimulating factor; P cell stimulating factor; P-cell-stimulating factor; PCSA
基因别名: BPA; Csfmu; HCGF; Il-3; Il3; MCGF; PSF
UniProt ID: (Mouse) P01586
Entrez Gene ID: (Mouse) 16187
