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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
酶联免疫吸附实验 (ELISA) |
Assay-Dependent | - |
对照试剂 (Ctrl) |
Assay-Dependent | - |
| 产品规格 | |
|---|---|
种属 |
Mouse |
表达系统 |
Insect |
氨基酸序列 |
Mouse IL-5, amino acids Met21-Gly133 (Accession # NM_010558) |
分子量 |
32-34 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>98% by SDS-PAGE |
偶联物 |
Unconjugated |
形式 |
Liquid |
纯化类型 |
Affinity chromatography |
内含物 |
0.09% sodium azide |
保存条件 |
-80°C |
Description: Recombinant mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.
Applications Reported: Recombinant mouse IL-5 is biologically active. The intended use of the Recombinant Mouse IL-5 Single-Use ELISA RSG Standard is as a standard for mouse IL-5 Ready-SET-Go! ELISA and mouse IL-5 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard described below.
Instructions for use: For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 5 µL of standard solution to 10 mL of assay diluent to prepare the top standard solution (500 pg/mL).For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 10 µL of standard solution to 10 mL of assay diluent to prepare the top standard solution (1000 pg/mL).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 µL in 1.0 mL) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested: This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IL-5 ELISA pair (TRFK5/TRFK4).
Source: Insect cells infected with baculovirus: amino acids Met 21-Gly 133 of mouse IL-5 accession # NM_010558.
Purity: >98% as determined by SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
基因别名: Il-5; Il5
UniProt ID: (Mouse) P04401
Entrez Gene ID: (Mouse) 16191
