Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
| 应用 | 建议稀释比 |
|---|---|
免疫组化 (IHC) |
Assay-Dependent |
免疫细胞化学 (ICC/IF) |
Assay-Dependent |
流式细胞分析 (Flow) |
5 µL (1 µg)/test |
对照试剂 (Ctrl) |
Assay-Dependent |
| 产品规格 | |
|---|---|
宿主/亚型 |
Mouse / IgG2b, kappa |
分类 |
Control |
类型 |
Isotype Control |
克隆号 |
eBMG2b |
偶联物 |
FITC
|
激发/发射光谱 |
查看光谱
|
形式 |
Liquid |
浓度 |
5 µL/Test |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_10598648 |
Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This mouse IgG2b isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.
Applications Tested: FITC Mouse IgG2b Isotype Control is offered in 2 formats:
- µg size: has been tested by flow cytometric analysis and can be used at the same concentration as the experimental antibody.- test size: has been pre-titrated and tested by intracellular flow cytometric analysis and can be used at test size: 5 µL (1 µg)/test per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
