应用 | 建议稀释比 | 已发表文章 |
---|---|---|
酶联免疫吸附实验 (ELISA) |
Assay-Dependent | - |
对照试剂 (Ctrl) |
Assay-Dependent | - |
其他PubMed (Misc) |
- | 查看1篇已发表文章 1篇已发表文章 |
产品规格 | |
---|---|
种属 |
Mouse |
已发表种属 |
Not Applicable |
表达系统 |
E. coli |
氨基酸序列 |
Mouse TNF-alpha, amino acids Leu80-Leu235 (Accession # NM_013693) |
分子量 |
17 kDa |
分类 |
Recombinant |
类型 |
Protein |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
Affinity chromatography |
内含物 |
no preservative |
保存条件 |
4° C |
Description: Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.
Applications Reported: Recombinant Mouse TNF alpha RSG ELISA Lyophilized Standard has been reported for use iin the Mouse TNF alpha ELISA Ready Set Go! (cat 88-7324).
Applications Tested: The intended use of the Mouse TNF alpha RSG ELISA Lyophilized Standard is as a standard for Mouse TNF alpha ELISA Ready-SET-Go!® (cat. 88-7324). Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard. Please refer to the Certificate of Analysis included with each ELISA set for instructions on the proper reconstitution and dilution of the lyophilized standard.
Note: These are single-use vials. Use one time within 4 hours of reconstitution and discard. Reconstitute and dilute as suggested in the Certificate of Analysis or recovery may not be accurate.
Source: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF alpha accession # NM_013693.
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: C-domain 1; C-domain 2; Cachectin; DADB-70P7.1; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha
基因别名: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2
UniProt ID: (Mouse) P06804
Entrez Gene ID: (Mouse) 21926