应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
其他PubMed (Misc) |
- | 查看4篇已发表文章 4篇已发表文章 |
产品规格 | |
---|---|
种属 |
Mouse |
已发表种属 |
Not Applicable |
表达系统 |
Yeast |
氨基酸序列 |
Mouse TNF-alpha, amino acids 80-235 (Accession # P06804) |
分子量 |
17.3 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>95% by SDS-PAGE |
活性 |
Determined by the cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
purified |
保存液 |
PBS |
内含物 |
no preservative |
保存条件 |
4° C |
The mature, soluble form of mouse TNF-alpha was expressed in Pichia pastoris, KM71H. GenBank Accession # P06804, amino acids 80 - 235. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: LRSSSQNSSD. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant mouse TNFa was measured by its cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: C-domain 1; C-domain 2; Cachectin; DADB-70P7.1; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha
基因别名: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2
UniProt ID: (Mouse) P06804
Entrez Gene ID: (Mouse) 21926