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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
功能检测 (FN) |
Assay-Dependent | - |
对照试剂 (Ctrl) |
Assay-Dependent | - |
其他PubMed (Misc) |
- | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Mouse |
已发表种属 |
Not Applicable |
表达系统 |
E. coli |
氨基酸序列 |
Mouse TPO, amino acids Glu22-Thr195 (Accession # NP_001166976.1) |
分子量 |
18.7 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>98% by SDS-PAGE and HPLC |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 ≤ 1.0 ng/mL; determined by the dose-dependent proliferation of human MO7e cells. This corresponds to a specific activity of at least 1.0x10e6 Units/mg. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
Affinity chromatography |
保存液 |
0.01M tris, pH 8.0 |
内含物 |
no preservative |
保存条件 |
-20°C |
Description: Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
Applications Reported: Recombinant human TPO is biologically active and can promote proliferation of human MO7e cells in culture.
Applications Tested: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0 ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg.
Source: E. coli (amino acids Glu22-Thr195; accession # NP_001166976.1).
Bioactivity: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0 ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg.
Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC.
Molecular Weight: 18.7 kDa.
Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water too a concentration of less than or equal to 0.5 mg/mL. This protein dissolves slowly. Do not vortex. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; ML; Myeloproliferative leukemia virus oncogene ligand; Thrombopoietin
基因别名: Mgdf; Ml; Mpllg; Thpo; Tpo
UniProt ID: (Mouse) P40226
Entrez Gene ID: (Mouse) 21832
