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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1:5,000-1:25,000 | - |
免疫组化 (IHC) |
1:200-1:1,000 | - |
免疫细胞化学 (ICC/IF) |
1:200-1:1,000 | - |
酶联免疫吸附实验 (ELISA) |
1:100 - 1:500 | - |
免疫沉淀 (IP) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
表达系统 |
Not Applicable |
类型 |
Protein |
偶联物 |
DyLight™ 488 |
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
purified |
保存液 |
PBS, pH 7.2, with 1% BSA |
内含物 |
0.02% sodium azide |
保存条件 |
4° C |
22832 contains 1 mL of protein in yellow colored liquid at a concentration of 1 mg/mL.
Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin-binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin binding proteins. The specific activity for biotin-binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
