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Invitrogen

OPA1 Polyclonal Antibody

2 Published Figures
2 References
View all (12) OPA1 antibodies

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OPA1 Polyclonal Antibody

Product Details

PA5-79771

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human OPA1 (919-955aa EDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEA).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL once reconstituted

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746886

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human MCF-7 whole cell, human PANC-1 whole cell, human PC-3 whole cell, human T47D whole cell, rat brain tissue, mouse brain tissue, mouse heart tissue, mouse NIH/3T3 whole cell. IHC: Mouse Liver tissue, Rat Kidney tissue, Human Lung Cancer tissue. ICC/IF: U20S cell. Flow: U20S cell.

Target Information

OPA1 is a dynamin-related GTPase that is critical for the maintenance of mitochondrial morphology and mtDNA. The most commonly associated phenotype with OPA1 mutations is heterozygous optic atrophy, a heterozygous dominant trait that causes reduced visual clarity and sometimes blindness. The disease usually begins in childhood and increases in severity throughout the life of affected individuals. Usually, this phenotype is attributed to the degeneration of optic nerve fibers. Interestingly, the same type of nerve degeneration seems to be partially causative of certain schizophrenia characteristics. OPA1 dysfunction also seems to be implicated in this case; mitochondrial networks associated with critical nerves seem to link schizophrenia and OPA1. The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: dynamin-like 120 kDa protein, mitochondrial; dynamin-like guanosine triphosphatase; GTPase; large GTP-binding protein; large GTPase; OPA1; largeG; mitochondrial dynamin-like GTPase; mitochondrial OPA1; optic atrophy 1 (autosomal dominant); optic atrophy 1 homolog; optic atrophy 1-like protein; optic atrophy protein 1; optic atrophy protein 1 homolog; RN protein; unnamed protein product

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Gene Aliases: 1200011N24Rik; BERHS; largeG; lilr3; MGM1; mKIAA0567; MTDPS14; MTDPS14A; MTDPS14B; NPG; NTG

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UniProt ID: (Human) O60313, (Mouse) P58281, (Rat) Q2TA68

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Entrez Gene ID: (Human) 4976, (Mouse) 74143, (Rat) 171116

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Function(s)
magnesium ion binding GTPase activity protein binding GTP binding microtubule binding phosphatidic acid binding GTPase-dependent fusogenic activity membrane bending activity cardiolipin binding kinase binding macromolecular complex binding
Process(es)
neural tube closure mitochondrion organization inner mitochondrial membrane organization visual perception mitochondrial fusion positive regulation of mitochondrial fusion regulation of mitochondrion organization positive regulation of neuron maturation peroxisome fission positive regulation of interleukin-17 production calcium ion transmembrane import into mitochondrion cristae formation negative regulation of apoptotic process GTP metabolic process positive regulation of insulin receptor signaling pathway intracellular distribution of mitochondria protein oligomerization positive regulation of dendritic spine morphogenesis membrane fusion negative regulation of release of cytochrome c from mitochondria cellular senescence membrane tubulation positive regulation of dendrite development positive regulation of cellular response to insulin stimulus negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway mitochondrial inner membrane fusion positive regulation of T-helper 17 cell lineage commitment negative regulation of intrinsic apoptotic signaling pathway mitochondrial fission axonal transport of mitochondrion response to muscle activity response to nutrient levels response to electrical stimulus retina development in camera-type eye cellular response to glucose stimulus cellular response to hypoxia cochlea development response to curcumin cellular response to L-glutamate
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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