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产品信息
PA5-50346
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1:200-1:1,000 | - |
免疫组化（石蜡） (IHC (P)) |
1:25-1:100 | - |
| 产品规格 | |
|---|---|
种属反应 |
Human |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Fusion protein corresponding to residues near the C terminal of human phosphatidylinositol-4-phosphate 5-kinase, type I, alpha |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
1.3 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
PBS, pH 7.4, with 40% glycerol |
内含物 |
0.05% sodium azide |
保存条件 |
-20°C |
RRID |
AB_2635799 |
产品详细信息
The antibody detects endogenous levels of total PIP5K1A protein.
靶标信息
Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase; phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase, type I, alpha; PI51A; PIP5K1-alpha; PIP5KIalpha; ptdIns(4)P-5-kinase 1 alpha
基因别名: PIP5K1A
UniProt ID: (Human) Q99755
Entrez Gene ID: (Human) 8394
分子生物学功能:
kinase
glycerophospholipid metabolic process
phosphatidylinositol biosynthetic process
phagocytosis
signal transduction
phospholipid biosynthetic process
fibroblast migration
regulation of phosphatidylinositol 3-kinase signaling
cell migration
keratinocyte differentiation
actin cytoskeleton reorganization
phosphatidylinositol phosphorylation
focal adhesion assembly
cell chemotaxis
protein targeting to plasma membrane
activation of GTPase activity
ruffle assembly
