Invitrogen

PP2A alpha/beta Polyclonal Antibody

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PP2A alpha/beta Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PP2A alpha/beta was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PP2A alpha/beta Polyclonal Antibody (Product # PA5-17980) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit-anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11078) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PP2A alpha/beta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PP2A alpha beta in paraffin embedded human kidney using a PP2A alpha beta polyclonal antibody (Product #PA5-17980) at a concentration of 10 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.

PP2A alpha/beta Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts of PC-3 (Lane 1), HEL 92.1.7 (Lane 2), K-562 (Lane 3), A549 (Lane 4), Jurkat (Lane 5) and A-431 (Lane 6). The blot was probed with Anti-PP2A alpha/beta Polyclonal Antibody (Product # PA5-17980, 2 µg/ml) and detected by chemiluminescence using Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/ml, 1:4000 dilution). A 36 kDa band corresponding to PP2A alpha/beta was observed across the cell lines tested. A regulatory subunit band around 65 kDa was observed across the cell lines tested.

产品信息

PA5-17980

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-3 µg/mL	-
免疫组化（石蜡） (IHC (P))	10µg/ml	-

产品规格
种属反应	Human, Mouse, Pig, Rat
宿主/亚型	Goat / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide sequence (EPHVTRRTPDYFL) corresponding to the C-terminus amino acids of PPP2CA / PPP2CB
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Antigen affinity chromatography
保存液	TBS, pH 7.3, with 0.5% BSA
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10986614

产品详细信息

This antibody is predicted to react with bovine, canine, mouse, porcine, rabbit and rat based on sequence homology.

靶标信息

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: PP2A-alpha; PP2A-beta; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; protein phosphatase 2, catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2, catalytic subunit, beta isoform; protein phosphatase 2, catalytic subunit, beta isozyme; protein phosphatase 2,catalytic subunit alpha isozyme; protein phosphatase 2A alpha subunit; protein phosphatase 2A beta subunit; protein phosphatase 2A catalytic alpha subunit; protein phosphatase 2A catalytic subunit alpha isoform; protein phosphatase 2A catalytic subunit beta isoform; protein phosphatase 2A catalytic subunit, beta isoform; protein phosphatase 2a, catalytic subunit, alpha isoform; protein phosphatase 2a, catalytic subunit, beta isoform; protein phosphatase type 2A catalytic subunit; protein phosphatase-2A-alpha; protein phosphatase-2A-beta; Replication protein C; RP C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform; testicular tissue protein Li 146

基因别名: AI115466; D8Ertd766e; PP2A; Pp2a1; Pp2a2; PP2Abeta; PP2Ac; PP2CA; PP2Calpha; PP2CB; PPP2CA; PPP2CB; R75353; RP-C

UniProt ID: (Human) P67775, (Pig) P67776, (Human) P62714, (Mouse) P63330, (Rat) P63331, (Rat) P62716, (Mouse) P62715

Entrez Gene ID: (Human) 5515, (Pig) 397656, (Human) 5516, (Pig) 397136, (Mouse) 19052, (Rat) 24672, (Rat) 24673, (Mouse) 19053

分子生物学功能: protein phosphatase

功能

参与通路

phosphoprotein phosphatase activity protein binding protein C-terminus binding metal ion binding GABA receptor binding protein serine/threonine phosphatase activity protein heterodimerization activity hydrolase activity enzyme binding protein kinase binding protein phosphatase binding protein domain specific binding protein complex binding protein kinase B binding ion channel binding protein dimerization activity tau protein binding protein phosphatase 2A binding protein antigen binding

PP2A alpha/beta Polyclonal Antibody

产品信息

PA5-17980

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

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