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Invitrogen
PREP Polyclonal Antibody
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PREP Antibody (PA5-115798) in ICC/IF
Immunocytochemistry analysis of PREP in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115798) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115798
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PREP(Accession P48147), corresponding to amino acid residues L168-D218.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total Prolyl Endopeptidase.
靶标信息
p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: dJ355L5.1 (prolyl endopeptidase); EC 3.4.21.26; MGC16060; PE; PE {ECO:0000250|UniProtKB:P23687}; Post-proline cleaving enzyme; post-proline cleaving enzyme {ECO:0000250|UniProtKB:P23687}; prep {ECO:0000312|RGD:620841}; Prolyl endopeptidase; prolyl endopeptidase {ECO:0000250|UniProtKB:P23687}; prolyl oligopeptidase; rPop
基因别名: AI047692; AI450383; D10Wsu136e; PE; PEP; Pop; PREP; rPop
UniProt ID: (Human) P48147, (Mouse) Q9QUR6, (Rat) O70196
Entrez Gene ID: (Human) 5550, (Mouse) 19072, (Rat) 83471
