Invitrogen

PTGES2 Polyclonal Antibody

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PTGES2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of PTGES2 in human heart muscle shows strong cytoplasmic positivity in myocytes. Samples were probed using a PTGES2 Polyclonal Antibody (Product # PA5-64984).

PTGES2 Antibody in Western Blot (WB) — Western blot was performed using Anti-PTGES2 Polyclonal Antibody (Product # PA5-64984) and a 33 kDa band corresponding to Prostaglandin E synthase 2 was observed across all cell lysates tested. Membrane enriched extracts (30 µg lysate) of SW480 (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), A549 (Lane 4), Caco-2 (Lane 5), LoVo (Lane 6) and MCF7 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). Uncharacterized bands (*) were observed at ~38 kDa, ~62 kDa in some cell lysates and all cell lysates displayed an uncharacterized band (*) at ~180 kDa.

产品信息

PA5-64984

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.04-0.4 µg/mL	-
免疫组化 (IHC)	1:50 - 1:200	-

产品规格
种属反应	Human
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant Human PTGES2 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	0.05 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.2, with 40% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2664793

产品详细信息

Immunogen sequence: AQDLHAERSAA QLSLSSRLQL TLYQYKTCPF CSKVRAFLDF HALPYQVVEV NPVRRAEIKF SSYRKVPILV AQEGESSQ

Highest antigen sequence identity to the following orthologs - mouse 89%, rat 91%.

靶标信息

The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ14038; gamma-interferon-activated transcriptional element-binding factor 1; GATE-binding factor 1; membrane-associated prostaglandin E synthase 2; Membrane-associated prostaglandin E synthase-2; MGC11289; Microsomal prostaglandin E synthase 2; microsomal prostaglandin E synthase-2; mPGE synthase-2; mPGES-2; Prostaglandin E synthase 2; Prostaglandin E synthase 2 truncated form; Prostaglandin-H(2) E-isomerase; RP11-395P17.2

基因别名: C9orf15; GBF-1; GBF1; mPGES-2; PGES2; PTGES2

UniProt ID: (Human) Q9H7Z7

Entrez Gene ID: (Human) 80142

分子生物学功能: reductase

功能

prostaglandin biosynthetic process cell redox homeostasis positive regulation of transcription, DNA-templated secretion oxidation-reduction process

参与通路

DNA binding protein binding electron carrier activity protein disulfide oxidoreductase activity lyase activity heme binding glutathione binding prostaglandin-E synthase activity

PTGES2 Polyclonal Antibody

产品信息

PA5-64984

免疫印迹 (WB)

免疫组化 (IHC)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

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