Invitrogen

Phospho-STAT1 (Tyr701) Monoclonal Antibody (ST1P-11A5)

Phospho-STAT1 (Tyr701) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-STAT1 (Tyr701) Antibody (33-3400) in IHC (P)

Immunohistochemistry analysis of Phospho-STAT1 (pTyr701) showing staining in the nucleus and cytoplasm of paraffin-embedded human cervical carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-m... View More

Phospho-STAT1 (Tyr701) Antibody in Western Blot (WB)

Phospho-STAT1 (Tyr701) Antibody in ChIP Assay (ChIP)

Phospho-STAT1 (Tyr701) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

33-3400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

查看20篇已发表文章 20篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

0.1-1.0 µg/mL

染色质免疫沉淀实验 (ChIP)

5 µg

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rhesus monkey

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

ST1P-11A5

抗原

Synthetic phospho peptide encompassing the conserved C-terminal site (Y701) of murine STAT1 protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533113

产品详细信息

This antibody reacts specifically with the tyrosine-701 phosphorylated form of STAT1 and does not exhibit appreciable cross-reactivity with corresponding tyrosine phosphorylated forms of other STAT proteins or with other endogenous phosphotyrosine-containing proteins. To confirm the exclusive recognition of tyrosine phosphorylated STAT1, Western blots were carried out on lysates of 293 cells transfected with a STAT1 expression vector together with a wild type or kinase ″dead″ JAK1 expression vector. In addition, recognition of endogenous tyrosine phosphorylated STAT1 was confirmed on Western blots with cell lysates derived from serum starved or EGF-stimulated A431 cells and on Western blots with IFNa-stimulated mouse embryo fibroblast lysates.

靶标信息

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Type I and III interferons shape the retinal cytokine network and barrier function in an in vitro model of ocular toxoplasmosis.

Frontiers in immunology

Geiller B,Greigert V,Hillenbrand CA,Gommenginger C,Beal L,Brunet J,Filisetti D,Villard O,Denis J,Pfaff AW

33-3400 was used in Western Blotting to show that stimulation with type I and III interferons did not limit parasite proliferation in retinal cells we tested.

Mon May 22 00:00:00 UTC 2023

The μ2 and λ1 Proteins of Mammalian Reovirus Modulate Early Events Leading to Induction of the Interferon Signaling Network.

Viruses

Després GD,Ngo K,Lemay G

33-3400 was used in Western Blotting to conclude that proteins involved in viral RNA synthesis are thus involved in this likely critical aspect of the ability of different reovirus variants to infect various cell types, and to discriminate between parental and transformed/cancer cells.

Sat Nov 26 00:00:00 UTC 2022

Signal transducer and activator of transcription (STAT) 1 and STAT3 are expressed in the human ovary and have Janus kinase 1-independent functions in the COV434 human granulosa cell line.

Reproduction, fertility, and development

Frost ER,Ford EA,Peters AE,Reed NL,McLaughlin EA,Baker MA,Lovell-Badge R,Sutherland JM

33-3400 was used in Western Blotting to implicate a conserved role for JAK/STAT signalling in human ovary development, warranting further investigation of this pathway in human granulosa cell function.

Sat Aug 01 00:00:00 UTC 2020

PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma.

Nature communications

Liu XD,Kong W,Peterson CB,McGrail DJ,Hoang A,Zhang X,Lam T,Pilie PG,Zhu H,Beckermann KE,Haake SM,Isgandrova S,Martinez-Moczygemba M,Sahni N,Tannir NM,Lin SY,Rathmell WK,Jonasch E

Published figure using Phospho-STAT1 (Tyr701) monoclonal antibody (Product # 33-3400) in Western Blot

Fri May 01 00:00:00 UTC 2020

PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma.

Nature communications

Liu XD,Kong W,Peterson CB,McGrail DJ,Hoang A,Zhang X,Lam T,Pilie PG,Zhu H,Beckermann KE,Haake SM,Isgandrova S,Martinez-Moczygemba M,Sahni N,Tannir NM,Lin SY,Rathmell WK,Jonasch E

Published figure using Phospho-STAT1 (Tyr701) monoclonal antibody (Product # 33-3400) in Western Blot

Fri May 01 00:00:00 UTC 2020

Analysis of interleukin-20 receptor complexes in trabecular meshwork cells and effects of cytokine signaling in anterior segment perfusion culture.

Molecular vision

Keller KE,Yang YF,Sun YY,Walter MR,Wirtz MK

Published figure using Phospho-STAT1 (Tyr701) monoclonal antibody (Product # 33-3400) in Western Blot

Wed Apr 22 00:00:00 UTC 2020

TWEAK blockade decreases atherosclerotic lesion size and progression through suppression of STAT1 signaling in diabetic mice.

Scientific reports

Fernández-Laso V,Sastre C,Méndez-Barbero N,Egido J,Martín-Ventura JL,Gómez-Guerrero C,Blanco-Colio LM

33-3400 was used in Western Blotting to demonstrate that TWEAK blockade delay plaque progression and alter plaque composition in diabetic atherosclerotic mice.

Thu Apr 27 00:00:00 UTC 2017

TWEAK blockade decreases atherosclerotic lesion size and progression through suppression of STAT1 signaling in diabetic mice.

Scientific reports

Fernández-Laso V,Sastre C,Méndez-Barbero N,Egido J,Martín-Ventura JL,Gómez-Guerrero C,Blanco-Colio LM

33-3400 was used in Western Blotting to demonstrate that TWEAK blockade delay plaque progression and alter plaque composition in diabetic atherosclerotic mice.

Thu Apr 27 00:00:00 UTC 2017

Vaccinia Virus Protein C6 Inhibits Type I IFN Signalling in the Nucleus and Binds to the Transactivation Domain of STAT2.

PLoS pathogens

Stuart JH,Sumner RP,Lu Y,Snowden JS,Smith GL

33-3400 was used in Immunocytochemistry-immunoflourescence to show that vaccinia virus strain Western Reserve protein C6, is a dual function protein that inhibits the cellular response to type I IFNs in addition to its published function as an inhibitor of IRF-3 activation.

Thu Dec 01 00:00:00 UTC 2016

Vaccinia Virus Protein C6 Inhibits Type I IFN Signalling in the Nucleus and Binds to the Transactivation Domain of STAT2.

PLoS pathogens

Stuart JH,Sumner RP,Lu Y,Snowden JS,Smith GL

Thu Dec 01 00:00:00 UTC 2016

PDGFRβ signalling regulates local inflammation and synergizes with hypercholesterolaemia to promote atherosclerosis.

Nature communications

He C,Medley SC,Hu T,Hinsdale ME,Lupu F,Virmani R,Olson LE

Published figure using Phospho-STAT1 (Tyr701) monoclonal antibody (Product # 33-3400) in Western Blot

Fri Jul 17 00:00:00 UTC 2015

STAT3 induction of miR-146b forms a feedback loop to inhibit the NF-κB to IL-6 signaling axis and STAT3-driven cancer phenotypes.

Science signaling

Xiang M,Birkbak NJ,Vafaizadeh V,Walker SR,Yeh JE,Liu S,Kroll Y,Boldin M,Taganov K,Groner B,Richardson AL,Frank DA

33-3400 was used in western blot to identify an epigenetic mechanism of crosstalk between STAT3 and NF-κB that regulates constitutive STAT3 activation in oncogenesis.

Tue Jan 28 00:00:00 UTC 2014

Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface.

Science (New York, N.Y.)

Nancy P,Tagliani E,Tay CS,Asp P,Levy DE,Erlebacher A

Fri Jun 08 00:00:00 UTC 2012

The JAK2/STAT3 signaling pathway is required for growth of CD44⁺CD24⁻ stem cell-like breast cancer cells in human tumors.

The Journal of clinical investigation

Marotta LL,Almendro V,Marusyk A,Shipitsin M,Schemme J,Walker SR,Bloushtain-Qimron N,Kim JJ,Choudhury SA,Maruyama R,Wu Z,Gönen M,Mulvey LA,Bessarabova MO,Huh SJ,Silver SJ,Kim SY,Park SY,Lee HE,Anderson KS,Richardson AL,Nikolskaya T,Nikolsky Y,Liu XS,Root DE,Hahn WC,Frank DA,Polyak K

33-3400 was used in Western Blotting to identify 15 genes required for cell growth and proliferation in CD44+CD24- human breast cancer cells.

Fri Jul 01 00:00:00 UTC 2011

PDlim2 selectively interacts with the PDZ binding motif of highly pathogenic avian H5N1 influenza A virus NS1.

PloS one

Yu J,Li X,Wang Y,Li B,Li H,Li Y,Zhou W,Zhang C,Wang Y,Rao Z,Bartlam M,Cao Y

Published figure using Phospho-STAT1 (Tyr701) monoclonal antibody (Product # 33-3400) in Western Blot

Mon Nov 28 00:00:00 UTC 2011

IL-27 directly restrains lung tumorigenicity by suppressing cyclooxygenase-2-mediated activities.

Journal of immunology (Baltimore, Md. : 1950)

Ho MY,Leu SJ,Sun GH,Tao MH,Tang SJ,Sun KH

Sun Nov 15 00:00:00 UTC 2009

Functional genomics highlights differential induction of antiviral pathways in the lungs of SARS-CoV-infected macaques.

PLoS pathogens

de Lang A,Baas T,Teal T,Leijten LM,Rain B,Osterhaus AD,Haagmans BL,Katze MG

33-3400 was used in immunocytochemistry and immunohistochemistry - paraffin section propose that induction of early IFN signaling may confer protection against severe acute respiratory syndrome coronavirus infection

Fri Aug 10 00:00:00 UTC 2007

Functional genomics highlights differential induction of antiviral pathways in the lungs of SARS-CoV-infected macaques.

PLoS pathogens

de Lang A,Baas T,Teal T,Leijten LM,Rain B,Osterhaus AD,Haagmans BL,Katze MG

Fri Aug 10 00:00:00 UTC 2007

IL-4 and IL-13 negatively regulate TNF-alpha- and IFN-gamma-induced beta-defensin expression through STAT-6, suppressor of cytokine signaling (SOCS)-1, and SOCS-3.

Journal of immunology (Baltimore, Md. : 1950)

Albanesi C,Fairchild HR,Madonna S,Scarponi C,De Pità O,Leung DY,Howell MD

33-3400 was used in ChIP Assay, ELISA, Immunohistochemistry (Paraffin), Western Blot to suggest that targeting the STAT-1-signaling pathway or suppressor of cytokine signaling expression enhances beta-defensin expression and represents a new therapeutic strategy for reduction of infection in human diseases associated with beta-defensin deficiency.

Sun Jul 15 00:00:00 UTC 2007

Identification of human STAT5-dependent gene regulatory elements based on interspecies homology.

The Journal of biological chemistry

Nelson EA,Walker SR,Li W,Liu XS,Frank DA

33-3400 was used in Chromatin immunoprecipitation to identify NCAM2 as both a STAT4- and STAT5-regulated gene.

Fri Sep 08 00:00:00 UTC 2006

Identification of human STAT5-dependent gene regulatory elements based on interspecies homology.

The Journal of biological chemistry

Nelson EA,Walker SR,Li W,Liu XS,Frank DA

33-3400 was used in Chromatin immunoprecipitation to identify NCAM2 as both a STAT4- and STAT5-regulated gene.

Fri Sep 08 00:00:00 UTC 2006

Signal regulatory protein alpha ligation induces macrophage nitric oxide production through JAK/STAT- and phosphatidylinositol 3-kinase/Rac1/NAPDH oxidase/H2O2-dependent pathways.

Molecular and cellular biology

Alblas J,Honing H,de Lavalette CR,Brown MH,Dijkstra CD,van den Berg TK

33-3400 was used in western blot to analyze the induction of macrophage nitric oxide production through JAK/STAT- and phosphatidylinositol 3-kinase/Rac1/NAPDH oxidase/H2O2-dependent pathways by signal regulatory protein alpha ligation

Mon Aug 01 00:00:00 UTC 2005

Double-stranded RNAs from the helminth parasite Schistosoma activate TLR3 in dendritic cells.

The Journal of biological chemistry

Aksoy E,Zouain CS,Vanhoutte F,Fontaine J,Pavelka N,Thieblemont N,Willems F,Ricciardi-Castagnoli P,Goldman M,Capron M,Ryffel B,Trottein F

33-3400 was used in western blot to analyze activation of TLR3 in dendritic cells by double-stranded RNAs from the helminth parasite Schistosoma

Fri Jan 07 00:00:00 UTC 2005

Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).

Oncogene

Duensing A,Medeiros F,McConarty B,Joseph NE,Panigrahy D,Singer S,Fletcher CD,Demetri GD,Fletcher JA

Thu May 13 00:00:00 UTC 2004

The protective effect of moderate hypothermia during intestinal ischemia-reperfusion is associated with modification of hepatic transcription factor activation.

Journal of pediatric surgery

Parkinson EJ,Townsend PA,Stephanou A,Latchman DS,Eaton S,Pierro A

33-3400 was used in western blot to study the mechanism of hypothermic protection during intestinal ischemia-reperfusion

Sat May 01 00:00:00 UTC 2004

Rapamycin inhibits the interleukin 10 signal transduction pathway and the growth of Epstein Barr virus B-cell lymphomas.

Cancer research

Nepomuceno RR,Balatoni CE,Natkunam Y,Snow AL,Krams SM,Martinez OM

33-3400 was used in western blot to report that rapamycin limits B-cell lymphoma growth while simultaneously providing immunosuppression to prevent graft rejection in patients and protection from EBV-associated post transplant lymphoproliferative disorder.

Fri Aug 01 00:00:00 UTC 2003

Constitutive activation of Jak/STAT proteins in Epstein-Barr virus-infected B-cell lines from patients with posttransplant lymphoproliferative disorder.

Transplantation

Nepomuceno RR,Snow AL,Robert Beatty P,Krams SM,Martinez OM

33-3400 was used in immunoprecipitation to investigate signaling pathways in posttransplant lymphoproliferative disease.

Thu Aug 15 00:00:00 UTC 2002

Activation of Stat3 in v-Src-transformed fibroblasts requires cooperation of Jak1 kinase activity.

The Journal of biological chemistry

Zhang Y,Turkson J,Carter-Su C,Smithgall T,Levitzki A,Kraker A,Krolewski JJ,Medveczky P,Jove R

Fri Aug 11 00:00:00 UTC 2000

生物信息学

蛋白别名: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT-1; stat1 alpha; Transcription factor ISGF-3 components p91/p84

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基因别名: 2010005J02Rik; AA408197; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91

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UniProt ID: (Human) P42224

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Entrez Gene ID: (Human) 6772, (Mouse) 20846

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific double-stranded DNA binding transcription factor activity, sequence-specific DNA binding signal transducer activity tumor necrosis factor receptor binding protein binding enzyme binding nuclear hormone receptor binding identical protein binding protein homodimerization activity cadherin binding involved in cell-cell adhesion DNA binding CCR5 chemokine receptor binding sequence-specific DNA binding protein phosphatase 2A binding DNA-binding transcription factor

参与通路

Phospho-STAT1 (Tyr701) Monoclonal Antibody (ST1P-11A5)

Phospho-STAT1 (Tyr701) Antibody (33-3400) in IHC (P)

产品信息

33-3400

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

染色质免疫沉淀实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

Phospho-STAT1 (Tyr701) Monoclonal Antibody (ST1P-11A5)

Phospho-STAT1 (Tyr701) Antibody (33-3400) in IHC (P)

产品信息

33-3400

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

染色质免疫沉淀 实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)