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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Rat |
表达系统 |
E. coli |
分子量 |
19 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>95% |
内毒素浓度 |
<1 EU/µg |
活性 |
EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394]As there is no international Rat IFN-alpha standard, units are determined with respect to the international reference standard for mouse interferon alpha/beta (mouse IFN-alpha/beta) provided by the National Institutes of Health. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
purified |
保存液 |
citric acid, pH 5, with NaCl, 0.125M trehalose |
内含物 |
no preservative |
保存条件 |
4° C |
Each blood donation used for interferon production tested negative for: HBsAg, HIV I-II, HCV and VDRL.
After receipt, this product should be kept at 4°C while lyophilized and at -70°C after reconstitution in sterile distilled water for retention of full activity. After reconstitution store at -70°C in aliquots. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Further dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: IFN-a; IFN-alpha 1b; IFNA 2; interferon alpha 1b; interferon alpha family, gene 2; interferon alpha-D; Interferon-alpha II; leIF D; RP11-354P17.2
Entrez Gene ID: (Rat) 298213
分子生物学功能:
interferon superfamily
