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| 应用 | 建议稀释比 |
|---|---|
流式细胞分析 (Flow) |
Assay-Dependent |
对照试剂 (Ctrl) |
Assay-Dependent |
| 产品规格 | |
|---|---|
宿主/亚型 |
Rat / IgM, kappa |
分类 |
Control |
类型 |
Isotype Control |
克隆号 |
eBRM |
偶联物 |
FITC
|
激发/发射光谱 |
查看光谱
|
形式 |
Liquid |
浓度 |
0.5 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2 |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_470017 |
Description: The monoclonal rat IgM, kappa is useful as an isotype control immunoglobulin.
Applications Reported: Rat IgM Isotype Control FITC has been reported for use in flow cytometric analysis.
Applications Tested: Rat IgM Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Immunoglobulin M is the third most common serum Ig and can exist as pentamer where all heavy chains are identical and all light chains are identical or as a monomer (e.g. found on B lymphocytes as B - cell receptors). The large pentameric structure allows for building of bridges between encountered epitopes on molecules that are too distant as to be connected by smaller IgG antibodies. IgM is built as the first antibody during an immune response and is responsible for agglutination and cytolytic reactions since its pentameric structure gives it 10 free antigen binding sites in theory and it posseses a high avidity. Due to conformational constraints among the 10 Fab portions, IgM only has a valence of 5. Additionally, IgM is not as versatile as IgG, but it is of vital importance in complement activation and agglutination. IgM is predominantly found in the lymph fluid and the blood and is a very effective neutralizing agent in the early stages of disease. Elevated levels can be a sign of recent infection or exposure to antigen.
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