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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Rhesus monkey |
表达系统 |
Yeast |
氨基酸序列 |
Rhesus macaque IL-18, amino acids 37-193 (Accession # AAK13416) |
分子量 |
18.3 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>95% by SDS-PAGE |
活性 |
ED50 = 20 - 40 ng/mL; determined by the dose-dependent induction of IFN-gamma production from KG-1 cells in the presence or recombinant mouse IL-12. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
purified |
保存液 |
PBS |
内含物 |
no preservative |
保存条件 |
4° C |
Mature rhesus macaque IL-18 was expressed in Pichia pastoris, X-33, GenBank Accession # AAK13416, amino acids 37 – 193. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: YFGKLESKLS. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant rhesus macaque IL-18 was determined by its ability to induce IFNg production from KG-1 cells in the presence of recombinant mouse IL-12. 270 pg/mL and 470 pg/mL of IFNg are induced by 20 ng/mL and 40 ng/mL of recombinant rhesus macaque IL-18, respectively.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: cytokine; il 18; ILN; Interleukin; interleukin 18 (interferon-gamma-inducing factor); interleukin-18 proprotein; Interleukin18; MGC12320
Entrez Gene ID: (Rhesus monkey) 574151
分子生物学功能:
cytokine
interleukin superfamily
intercellular signal molecule
