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Invitrogen
Rhodopsin Monoclonal Antibody (1D4)
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Rhodopsin Antibody (MA1-722) in IHC (P)
Immunohistochemistry analysis of Rhodopsin showing staining in the nucleus of paraffin-embedded mouse retinal tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature,... View More
产品信息
MA1-722
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Mouse,
Rat
已发表种属
Bovine,
C. elegans,
Fruit fly,
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
1D4
抗原
Bovine rhodopsin.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-722 detects rhodopsin from human, mouse and bovine retinal samples. Clone 1D4 has also been used successfully in Zebrafish. It does not recognize rhodopsin in zebrafish rods, but instead labels long double cone outer segments that express red opsin (Yin, J. et al, 2012).
MA1-722 has been successfully used in Western blot, IHC (P), immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~40 kDa protein representing rhodopsin from Sf9 cells expressing the bovine gene. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.
The MA1-722 immunogen is bovine rhodopsin. The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.
靶标信息
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; Rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rhodopsin (retinitis pigmentosa 4, autosomal dominant); Rod Opsin; RP 4
基因别名: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4
UniProt ID: (Bovine) P02699, (Human) P08100, (Rat) P51489, (Mouse) P15409
Entrez Gene ID: (Bovine) 509933, (Human) 6010, (Rat) 24717, (Mouse) 212541
G-protein alpha-subunit binding
opsin binding
guanyl-nucleotide exchange factor activity
11-cis retinal binding
protein binding
G-protein coupled photoreceptor activity
zinc ion binding
identical protein binding
arrestin family protein binding
G-protein coupled receptor activity
retinal binding
metal ion binding
photoreceptor activity
spectrin binding
signal transducer activity
G-protein coupled receptor
protein phosphorylation
visual perception
phototransduction, visible light
response to light stimulus
absorption of visible light
rhodopsin mediated signaling pathway
protein-chromophore linkage
photoreceptor cell maintenance
retina development in camera-type eye
retinoid metabolic process
G-protein coupled receptor signaling pathway
red, far-red light phototransduction
regulation of rhodopsin mediated signaling pathway
phototransduction
detection of light stimulus
sensory perception of light stimulus
cellular response to light stimulus
signal transduction
response to stimulus
