Invitrogen

SLC7A11 Recombinant Polyclonal Antibody (3HCLC)

Product Image Gallery

SLC7A11 Antibody in Immunocytochemistry (ICC/IF) — For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous xCT/ SLC7A11 using xCT SLC7A11 Recombinant Rabbit Polyclonal Antibody (Product # 711589, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of xCT/ SLC7A11 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of xCT/ SLC7A11 in the membrane/cytoplasm. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

SLC7A11 Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts and tissue extracts (30 µg lysate) of SH-SY5Y (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), K-562 (Lane 4), Mouse Stomach (Lane 5) and U-87 MG (Lane 6). The blots were probed with Anti- xCT/ SLC7A11 Recombinant Rabbit Polyclonal Antibody (Product # 711589, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 55 kDa band corresponding to xCT/ SLC7A11 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

产品信息

711589

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-2 µg/mL	查看1篇已发表文章 1篇已发表文章
免疫细胞化学 (ICC/IF)	2 µg/mL	-

产品规格
种属反应	Human, Mouse
已发表种属	Human
宿主/亚型	Rabbit / IgG
分类	Recombinant Polyclonal
类型	Antibody
克隆号	3HCLC
抗原	Peptides corresponding to Human SLC7A11 (aa 30-43, 214-230)
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Protein A
保存液	PBS, pH 7.4
内含物	0.09% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2633188

产品详细信息

This antibody is predicted to react with Monkey, Horse, Pig and Sheep

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

SLC7A11 (also known as XCT) is a member of a hetromeric, sodium-independent, anionic amino acid transport system. It is highly specific for cysteine and glutamate. SLC7A11 is a mediator of Kaposi sarcoma-associated herpes virus fusion and entry into cells. Increased expression of this protein in primary gliomas is associated with neuronal cell death.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CD8⁺ T cells regulate tumour ferroptosis during cancer immunotherapy.

Nature

Wang W,Green M,Choi JE,Gijón M,Kennedy PD,Johnson JK,Liao P,Lang X,Kryczek I,Sell A,Xia H,Zhou J,Li G,Li J,Li W,Wei S,Vatan L,Zhang H,Szeliga W,Gu W,Liu R,Lawrence TS,Lamb C,Tanno Y,Cieslik M,Stone E,Georgiou G,Chan TA,Chinnaiyan A,Zou W

711589 was used in Western Blotting to show that immunotherapy-activated CD8+ T cells enhance ferroptosis-specific lipid peroxidation in tumour cells and that increased ferroptosis contributes to the anti-tumour efficacy of immunotherapy.

Wed May 01 00:00:00 EDT 2019

生物信息学

蛋白别名: Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1; cysteine/glutamate transporter; Cystine/glutamate transporter; sodium independent anionic amino acid transport system; solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11; Solute carrier family 7 member 11; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11; xCT

基因别名: 9930009M05Rik; AI451155; CCBR1; SLC7A11; sut; xCT

UniProt ID: (Human) Q9UPY5, (Mouse) Q9WTR6

Entrez Gene ID: (Human) 23657, (Mouse) 26570

分子生物学功能: transporter