Invitrogen

SNAP25 Monoclonal Antibody (SP12)

SNAP25 Antibody in Immunocytochemistry (ICC/IF)

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SNAP25 Antibody (MA5-17609) in ICC/IF

Immunofluorescence analysis of Synaptosomal-associated protein 25 was performed using 70% confluent log phase PC-12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SNAP25 Monoclonal Antibody (SP12) (Product # MA5-17609) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody... View More

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产品信息

MA5-17609

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

1:2,000

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

SP12

抗原

Synaptic preparation of post mortem human brain

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538999

产品详细信息

MA5-17609 targets SNAP-25 in ELISA, FACS, and WB applications and shows reactivity with Human samples.

The MA5-17609 immunogen is human Synaptobrevin 1 (aa 1-118) and Synaptobrevin 2(aa 33-96).

靶标信息

SNAP25 is a t-SNARE involved in the molecular regulation of neurotransmitter release. It may play a role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. It also regulates plasma membrane recycling through its interaction with CENPF. Mutations in the gene can results in myasthenic syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Investigating the Impact of IL6 on Insulin Secretion: Evidence from INS-1 Cells, Human Pancreatic Islets, and Serum Analysis.

Cells

Taneera J,Khalique A,Mohammed AK,Mussa BM,Sulaiman N,Abu-Gharbieh E,El-Huneidi W,Saber-Ayad MM

Published figure using SNAP25 monoclonal antibody (Product # MA5-17609) in Western Blot

Mon Apr 15 00:00:00 UTC 2024

Expression Silencing of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 Conferred Its Role in Pancreatic β-Cell Physiology and Insulin Secretion.

Metabolites

Saeed R,Mohammed AK,Saleh SE,Aboshanab KM,Aboulwafa MM,Taneera J

MA5-17609 was used in Western Blotting to report that siRNA silencing of Pdx1 resulted in the downregulation of MAPK8IP1 expression in INS-1 cells.

Mon Feb 20 00:00:00 UTC 2023

Expression Silencing of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 Conferred Its Role in Pancreatic β-Cell Physiology and Insulin Secretion.

Metabolites

Saeed R,Mohammed AK,Saleh SE,Aboshanab KM,Aboulwafa MM,Taneera J

MA5-17609 was used in Western Blotting to report that siRNA silencing of Pdx1 resulted in the downregulation of MAPK8IP1 expression in INS-1 cells.

Mon Feb 20 00:00:00 UTC 2023

Heart failure-induced cognitive dysfunction is mediated by intracellular Ca²⁺ leak through ryanodine receptor type 2.

Nature neuroscience

Dridi H,Liu Y,Reiken S,Liu X,Argyrousi EK,Yuan Q,Miotto MC,Sittenfeld L,Meddar A,Soni RK,Arancio O,Lacampagne A,Marks AR

MA5-17609 was used in Western Blot to propose that HF is a systemic illness driven by intracellular Ca2+ leak that includes cardiogenic dementia.

Tue Aug 01 00:00:00 UTC 2023

Structural Basis for Substrate Recognition by the Ankyrin Repeat Domain of Human DHHC17 Palmitoyltransferase.

Structure (London, England : 1993)

Verardi R,Kim JS,Ghirlando R,Banerjee A

Published figure using SNAP25 monoclonal antibody (Product # MA5-17609) in Western Blot

Tue Sep 05 00:00:00 UTC 2017

生物信息学

蛋白别名: ALMS1; blind drunk; CG17676; CG17884; CG40452; Dmel CG40452; Dmel_CG40452; FLJ23079; HGNC:11132; resistance to inhibitors of cholinesterase 4 homolog; SNAP 25; SNAP-25; snp25; SUP; Super protein; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; synaptosomal-associated protein, 25 kDa; synaptosomal-associated protein, 25kDa; synaptosome associated protein 25kDa

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基因别名: bA416N4.2; Bdr; CMS18; dJ1068F16.2; GENA70; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SNAP-25a; SNAP-25B; SNAP25; sp; SUP

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UniProt ID: (Human) P60880, (Rat) P60881, (Mouse) P60879

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Entrez Gene ID: (Human) 6616, (Rat) 25012, (Mouse) 20614

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功能