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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TNF alpha Antibodies

          Invitrogen

          TNF alpha Monoclonal Antibody (MP6-XT22), Alexa Fluor™ 488, eBioscience™

          78个已发表图片
          68 篇参考文献
          查看其他158个TNF alpha抗体

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          说明书
          实验方案
          问题与答案
          技术支持

          Cite TNF alpha Monoclonal Antibody (MP6-XT22), Alexa Fluor™ 488, eBioscience™

          • 抗体检测数据 (1)
          • 已发表图片 (78)
          TNF alpha Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          TNF alpha Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          图: 1 / 79

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          TNF alpha Antibody (53-7321-82) in Flow

          Intracellular staining of restimulated C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse TNF alpha Alexa Fluor® 488 (filled histogram). Total cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Immunohistochemistry (IHC)
          TNF alpha Antibody in Immunohistochemistry (IHC)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in ELISA (ELISA)
          TNF alpha Antibody in Neutralization (Neu)
          TNF alpha Antibody in Neutralization (Neu)

          产品信息

          53-7321-82

          应用
          建议稀释比
          已发表文章

          免疫组化 (IHC)

          -
          查看2篇已发表文章 2篇已发表文章

          流式细胞分析 (Flow)

          0.12 µg/test
          查看64篇已发表文章 64篇已发表文章

          酶联免疫吸附实验 (ELISA)

          -
          查看1篇已发表文章 1篇已发表文章

          中和实验 (Neu)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Mouse

          已发表种属

          Mouse

          宿主/亚型

          Rat / IgG1, kappa

          推荐的同型对照

          Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          MP6-XT22

          偶联物

          Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
          • Unconjugated
          • Alexa Fluor 561
          • Alexa Fluor 660 
          • APC 
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 786
          • eFluor 450
          • FITC
          • Functional Grade
          • PE 
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Request custom conjugation

          激发/发射光谱

          499/520 nm 查看光谱 spectra

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4°C, store in dark, DO NOT FREEZE!

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_469936

          产品详细信息

          Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

          Applications Reported: The MP6-XT22 antibody has been reported for use in ELISA, IHC, neutralization, and immunofluorescent intracellular staining with flow cytometric analysis. Fluorochrome conjugated MP6-XT22 antibody is recommended for use in intracellular staining.

          Applications Tested: This MP6-XT22 antibody has been tested by intracellular immunofluorescent staining and flow cytometric analysis of activated splenocytes cultured in the presence of monensin. This can be used at less than or equal to 0.12 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          生物信息学

          蛋白别名: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

          查看更多 收起

          基因别名: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2

          查看更多 收起

          UniProt ID: (Mouse) P06804

          查看更多 收起

          Entrez Gene ID: (Mouse) 21926

          查看更多 收起

          功能
          protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding
          参与通路
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPKKK activity activation of MAPK activity cell activation positive regulation of cytokine production regulation of protein phosphorylation positive regulation of protein phosphorylation negative regulation of L-glutamate transport chronic inflammatory response to antigenic stimulus negative regulation of cytokine secretion involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin glucose metabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process obsolete transformed cell apoptotic process defense response inflammatory response immune response humoral immune response signal transduction JNK cascade multicellular organism development cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to virus organ morphogenesis positive regulation of gene expression negative regulation of gene expression negative regulation of alkaline phosphatase activity calcium-mediated signaling extracellular matrix organization osteoclast differentiation sequestering of triglyceride positive regulation of protein complex assembly positive regulation of fever generation lipopolysaccharide-mediated signaling pathway negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-18 production positive regulation of interleukin-6 production receptor biosynthetic process positive regulation of peptidyl-serine phosphorylation tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion regulation of cell proliferation positive regulation of NF-kappaB import into nucleus defense response to bacterium positive regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of protein complex disassembly positive regulation of protein complex disassembly positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity positive regulation of neuron apoptotic process negative regulation of growth of symbiont in host negative regulation of viral genome replication positive regulation of chemokine biosynthetic process cellular extravasation positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of myoblast differentiation negative regulation of osteoblast differentiation regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of action potential positive regulation of cell adhesion positive regulation of mitotic nuclear division positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import positive regulation of JNK cascade positive regulation of smooth muscle cell proliferation regulation of protein secretion positive regulation of cytokine secretion positive regulation of inflammatory response positive regulation of phagocytosis regulation of insulin secretion positive regulation of synaptic transmission defense response to Gram-positive bacterium leukocyte migration leukocyte tethering or rolling detection of mechanical stimulus involved in sensory perception of pain negative regulation of lipid catabolic process regulation of immunoglobulin secretion positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein transport response to glucocorticoid positive regulation of NFAT protein import into nucleus positive regulation of hair follicle development positive regulation of protein kinase B signaling positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of branching involved in lung morphogenesis cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to nicotine cellular response to organic cyclic compound positive regulation of podosome assembly apoptotic signaling pathway extrinsic apoptotic signaling pathway necroptotic signaling pathway positive regulation of protein localization to cell surface positive regulation of chemokine (C-X-C motif) ligand 2 production regulation of reactive oxygen species metabolic process negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.
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