Invitrogen

TNF alpha Monoclonal Antibody (MP6-XT22), Alexa Fluor™ 488, eBioscience™

TNF alpha Antibody in Flow Cytometry (Flow)

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TNF alpha Antibody (53-7321-82) in Flow

Intracellular staining of restimulated C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse TNF alpha Alexa Fluor® 488 (filled histogram). Total cells were used for analysis.

TNF alpha Antibody in Immunohistochemistry (IHC)

TNF alpha Antibody in Neutralization (Neu)

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产品信息

53-7321-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

0.12 µg/test

查看64篇已发表文章 64篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

中和实验 (Neu)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MP6-XT22

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_469936

产品详细信息

Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported: The MP6-XT22 antibody has been reported for use in ELISA, IHC, neutralization, and immunofluorescent intracellular staining with flow cytometric analysis. Fluorochrome conjugated MP6-XT22 antibody is recommended for use in intracellular staining.

Applications Tested: This MP6-XT22 antibody has been tested by intracellular immunofluorescent staining and flow cytometric analysis of activated splenocytes cultured in the presence of monensin. This can be used at less than or equal to 0.12 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

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Ly6C-high monocytes alleviate brain injury in experimental subarachnoid hemorrhage in mice.

Journal of neuroinflammation

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Tumor-secreted IFI35 promotes proliferation and cytotoxic activity of CD8⁺ T cells through PI3K/AKT/mTOR signaling pathway in colorectal cancer.

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Conditional Knockout of Hypoxia-Inducible Factor 1-Alpha in Tumor-Infiltrating Neutrophils Protects against Pancreatic Ductal Adenocarcinoma.

International journal of molecular sciences

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Infiltrating myeloid cell-derived properdin markedly promotes microglia-mediated neuroinflammation after ischemic stroke.

Journal of neuroinflammation

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Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection.

International journal of molecular sciences

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Airway Prevotella promote TLR2-dependent neutrophil activation and rapid clearance of Streptococcus pneumoniae from the lung.

Nature communications

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Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

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Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8⁺ T lymphocytes.

Nature communications

Tan TCJ,Kelly V,Zou X,Wright D,Ly T,Zamoyska R

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Intranasal delivery of a small-molecule ErbB inhibitor promotes recovery from acute and late-stage CNS inflammation.

JCI insight

Linnerbauer M,Lößlein L,Vandrey O,Tsaktanis T,Beer A,Naumann UJ,Panier F,Beyer T,Nirschl L,Kuramatsu JB,Winkler J,Quintana FJ,Rothhammer V

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Interleukin-37 protects against acinar cell pyroptosis in acute pancreatitis.

JCI insight

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Circular RNA cancer vaccines drive immunity in hard-to-treat malignancies.

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Z. morio Hemolymph Relieves E. coli-Induced Mastitis by Inhibiting Inflammatory Response and Repairing the Blood-Milk Barrier.

International journal of molecular sciences

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Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the brain.

eLife

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53-7321-82 was used in Flow cytometry/Cell sorting to reveal that CNS lymphatic drainage supports the development of peripheral T cell responses against T. gondii but remains dispensable for immune protection of the brain.

Wed Dec 21 00:00:00 UTC 2022

COVID-19 treatments and pathogenesis including anosmia in K18-hACE2 mice.

Nature

Zheng J,Wong LR,Li K,Verma AK,Ortiz ME,Wohlford-Lenane C,Leidinger MR,Knudson CM,Meyerholz DK,McCray PB,Perlman S

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The ubiquitin ligase MDM2 sustains STAT5 stability to control T cell-mediated antitumor immunity.

Nature immunology

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Mapping and role of T cell response in SARS-CoV-2-infected mice.

The Journal of experimental medicine

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USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Nature communications

Yang Z,Xu G,Wang B,Liu Y,Zhang L,Jing T,Tang M,Xu X,Jiao K,Xiang L,Fu Y,Tang D,Zhang X,Jin W,Zhuang G,Zhao X,Liu Y

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Scientific reports

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The RNase MCPIP3 promotes skin inflammation by orchestrating myeloid cytokine response.

Nature communications

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GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure.

Cell death & disease

Yu H,Yang W,Huang J,Miao X,Wang B,Ren X,Gu Y,Wang Q,Ding X,Guo X,Qian F,Zhang Y,Xu H,Zheng L,Jin M

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Rate of Freeze Impacts the Survival and Immune Responses Post Cryoablation of Melanoma.

Frontiers in immunology

Yakkala C,Dagher J,Sempoux C,Chiang CL,Denys A,Kandalaft LE,Koppolu B,Duran R

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Influenza NG-34 T cell conserved epitope adjuvanted with CAF01 as a possible influenza vaccine candidate.

Veterinary research

Sisteré-Oró M,Pedersen GK,Córdoba L,López-Serrano S,Christensen D,Darji A

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Influenza NG-34 T cell conserved epitope adjuvanted with CAF01 as a possible influenza vaccine candidate.

Veterinary research

Sisteré-Oró M,Pedersen GK,Córdoba L,López-Serrano S,Christensen D,Darji A

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Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2.

BioMed research international

Zhao K,Tian Y,Wang J,Chen C,Pan B,Yan Z,Zhu S,Xu K

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Fgr contributes to hemorrhage-induced thalamic pain by activating NF-κB/ERK1/2 pathways.

JCI insight

Huang T,Fu G,Gao J,Zhang Y,Cai W,Wu S,Jia S,Xia S,Bachmann T,Bekker A,Tao YX

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Immunohistochemistry

Thu Oct 15 00:00:00 UTC 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

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Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

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Fri Jan 10 00:00:00 UTC 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

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The overexpression of TDP-43 in astrocytes causes neurodegeneration via a PTP1B-mediated inflammatory response.

Journal of neuroinflammation

Lee S,Kim S,Kang HY,Lim HR,Kwon Y,Jo M,Jeon YM,Kim SR,Kim K,Ha CM,Lee S,Kim HJ

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Neutralization

Wed Oct 14 00:00:00 UTC 2020

Mucosal-associated invariant T cells promote inflammation and intestinal dysbiosis leading to metabolic dysfunction during obesity.

Nature communications

Toubal A,Kiaf B,Beaudoin L,Cagninacci L,Rhimi M,Fruchet B,da Silva J,Corbett AJ,Simoni Y,Lantz O,Rossjohn J,McCluskey J,Lesnik P,Maguin E,Lehuen A

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Fri Jul 24 00:00:00 UTC 2020

Dissecting the phenotypic and functional heterogeneity of mouse inflammatory osteoclasts by the expression of Cx3cr1.

eLife

Madel MB,Ibáñez L,Ciucci T,Halper J,Rouleau M,Boutin A,Hue C,Duroux-Richard I,Apparailly F,Garchon HJ,Wakkach A,Blin-Wakkach C

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Wed May 13 00:00:00 UTC 2020

Tolerogenic Immunomodulation by PEGylated Antigenic Peptides.

Frontiers in immunology

Pfeil J,Simonetti M,Lauer U,Volkmer R,von Thülen B,Durek P,Krähmer R,Leenders F,Hamann A,Hoffmann U

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Thu Apr 29 00:00:00 UTC 2021

Enhanced Therapeutic Efficacy of a Novel Oncolytic Herpes Simplex Virus Type 2 Encoding an Antibody Against Programmed Cell Death 1.

Molecular therapy oncolytics

Zhu Y,Hu X,Feng L,Yang Z,Zhou L,Duan X,Cheng S,Zhang W,Liu B,Zhang K

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Fri Dec 20 00:00:00 UTC 2019

Maternal vaccination and protective immunity against Zika virus vertical transmission.

Nature communications

Shan C,Xie X,Luo H,Muruato AE,Liu Y,Wakamiya M,La JH,Chung JM,Weaver SC,Wang T,Shi PY

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Thu Dec 12 00:00:00 UTC 2019

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.

Frontiers in immunology

Xu J,Pei S,Wang Y,Liu J,Qian Y,Huang M,Zhang Y,Xiao Y

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Wed Oct 14 00:00:00 UTC 2020

Interleukin 1 receptor (IL-1R1) activation exacerbates toxin-induced acute kidney injury.

American journal of physiology. Renal physiology

Privratsky JR,Zhang J,Lu X,Rudemiller N,Wei Q,Yu YR,Gunn MD,Crowley SD

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Sat Sep 01 00:00:00 UTC 2018

Targeting macrophage and microglia activation with colony stimulating factor 1 receptor inhibitor is an effective strategy to treat injury-triggered neuropathic pain.

Molecular pain

Lee S,Shi XQ,Fan A,West B,Zhang J

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Mon Oct 22 00:00:00 UTC 2018

Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.

Nature communications

Kim DH,Park HJ,Lim S,Koo JH,Lee HG,Choi JO,Oh JH,Ha SJ,Kang MJ,Lee CM,Lee CG,Elias JA,Choi JM

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Mon Feb 05 00:00:00 UTC 2018

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Thu Sep 06 00:00:00 UTC 2018

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Cell metabolism

Thwe PM,Pelgrom LR,Cooper R,Beauchamp S,Reisz JA,D'Alessandro A,Everts B,Amiel E

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Tue Sep 05 00:00:00 UTC 2017

A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.

The Journal of clinical investigation

Berlato C,Khan MN,Schioppa T,Thompson R,Maniati E,Montfort A,Jangani M,Canosa M,Kulbe H,Hagemann UB,Duncan AR,Fletcher L,Wilkinson RW,Powles T,Quezada SA,Balkwill FR

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Wed Mar 01 00:00:00 UTC 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Mon Jun 05 00:00:00 UTC 2017

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Wed Nov 20 00:00:00 UTC 2019

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Wed Oct 11 00:00:00 UTC 2017

The TLR7 agonist imiquimod induces anti-cancer effects via autophagic cell death and enhances anti-tumoral and systemic immunity during radiotherapy for melanoma.

Oncotarget

Cho JH,Lee HJ,Ko HJ,Yoon BI,Choe J,Kim KC,Hahn TW,Han JA,Choi SS,Jung YM,Lee KH,Lee YS,Jung YJ

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Tue Apr 11 00:00:00 UTC 2017

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Thu Aug 03 00:00:00 UTC 2017

Toxoplasma Co-infection Prevents Th2 Differentiation and Leads to a Helminth-Specific Th1 Response.

Frontiers in cellular and infection microbiology

Ahmed N,French T,Rausch S,Kühl A,Hemminger K,Dunay IR,Steinfelder S,Hartmann S

53-7321 was used in Flow cytometry/Cell sorting to conclude a previous T. gondii infection limits a helminth-specific Th2 immune response while promoting a shift toward a Th1-type immune response.

Mon Apr 02 00:00:00 UTC 2018

Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion.

The Journal of experimental medicine

Hu Y,Kim JH,He K,Wan Q,Kim J,Flach M,Kirchhausen T,Vortkamp A,Winau F

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Mon Nov 14 00:00:00 UTC 2016

Reprogramming the immunological microenvironment through radiation and targeting Axl.

Nature communications

Aguilera TA,Rafat M,Castellini L,Shehade H,Kariolis MS,Hui AB,Stehr H,von Eyben R,Jiang D,Ellies LG,Koong AC,Diehn M,Rankin EB,Graves EE,Giaccia AJ

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Fri Dec 23 00:00:00 UTC 2016

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks.

Cell

Parnas O,Jovanovic M,Eisenhaure TM,Herbst RH,Dixit A,Ye CJ,Przybylski D,Platt RJ,Tirosh I,Sanjana NE,Shalem O,Satija R,Raychowdhury R,Mertins P,Carr SA,Zhang F,Hacohen N,Regev A

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Thu Jul 30 00:00:00 UTC 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Fri Jul 10 00:00:00 UTC 2015

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Fri Mar 11 00:00:00 UTC 2016

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Mon Jan 05 00:00:00 UTC 2015

Evaluation of Lassa virus vaccine immunogenicity in a CBA/J-ML29 mouse model.

Vaccine

Goicochea MA,Zapata JC,Bryant J,Davis H,Salvato MS,Lukashevich IS

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Flow Cytometry

Tue Feb 14 00:00:00 UTC 2012

Tissue specific deletion of inhibitor of kappa B kinase 2 with OX40-Cre reveals the unanticipated expression from the OX40 locus in skin epidermis.

PloS one

Cornish GH,Tung SL,Marshall D,Ley S,Seddon BP

Published figure using TNF alpha monoclonal antibody (Product # 53-7321-82) in Immunofluorescence

Fri Jun 29 00:00:00 UTC 2012

生物信息学

蛋白别名: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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基因别名: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2

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UniProt ID: (Mouse) P06804

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Entrez Gene ID: (Mouse) 21926

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功能

protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding

参与通路