Invitrogen

TRIM65 Polyclonal Antibody

查看其他7个TRIM65抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (2)

TRIM65 Antibody in Immunocytochemistry (ICC/IF)

TRIM65 Antibody in Immunocytochemistry (ICC/IF)

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TRIM65 Antibody (PA5-98519) in ICC/IF

Immunofluorescent analysis of TRIM65 in U251 cells using a TRIM65 polyclonal antibody (Product # PA5-98519) at a dilution of 1:50. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

TRIM65 Antibody in Immunocytochemistry (ICC/IF)

TRIM65 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TRIM65 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of TRIM65 in U251 cells using a TRIM65 polyclonal antibody (Product # PA5-98519) at a dilution of 1:50. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

TRIM65 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of TRIM65 in paraffin embedded human endometrial cancer using a TRIM65 polyclonal antibody (Product # PA5-98519) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen image

TRIM65 Polyclonal Antibody

Immunogen Image TRIM65 Polyclonal Antibody

产品信息

PA5-98519

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-Dependent

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Tripartite motif-containing protein 65 protein (290-517AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.21 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

TRIM65 is a protein coding gene and gene ontology (GO) includes cytosol, nucleoplasm, nucleus, positive regulation of autophagy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: E3 ubiquitin-protein ligase TRIM65; Tripartite motif-containing protein 65

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基因别名: 4732463G12Rik; TRIM65

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UniProt ID: (Human) Q6PJ69

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Entrez Gene ID: (Human) 201292

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zinc ion binding

positive regulation of autophagy