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Invitrogen
TSH Receptor Monoclonal Antibody (49)
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TSH Receptor Antibody (MA3-218) in ICC/IF
Immunofluorescence analysis of Thyrotropin Receptor was performed using 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TSH Receptor (49) Mouse Monoclonal Antibody (Product # MA3-218) at 1:250 dilution in 0.1% BSA and incu... View More
产品信息
MA3-218
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Pig
已发表种属
C. elegans
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
49
抗原
Purified extracellular domain of recombinant human thyrotropin receptor.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-218 detects both glycosylated and nonglycosylated thyroid stimulating hormone receptor (TSHR) from porcine tissues as well as recombinant human TSHR.
MA3-218 has been successfully used in Western blot, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects a 50 kDa and a 63 kDa protein representing unglycosylated and glycosylated TSHR respectively, in transfected Sf9 cell extract. By ELISA MA3-218 has been used in TSH competitive inhibition studies.
The MA3-218 antigen is purified extracellular domain of recombinant human thyrotropin receptor. This antibody recognizes an epitope between amino acid residues 277-296 of human TSHR.
靶标信息
TSH Receptor gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC141920; seven transmembrane helix receptor; thyroid stimulating hormone receptor, isoform 2; Thyroid-stimulating hormone receptor; Thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; TSH-R; TSHR; seven transmembrane domain G protein-coupled receptor
基因别名: CHNG1; hTSHR-I; LGR3; TSHR
UniProt ID: (Pig) Q8SPP9, (Human) P16473
Entrez Gene ID: (Pig) 397560, (Human) 7253
cell surface receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
thyroid-stimulating hormone signaling pathway
cellular response to glycoprotein
cellular response to thyrotropin-releasing hormone
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
activation of adenylate cyclase activity
cell-cell signaling
nervous system development
positive regulation of cell proliferation
hormone-mediated signaling pathway
