Invitrogen

TYK2 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

TYK2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

TYK2 Antibody (720124) in ICC/IF

Immunofluorescence was performed on fixed and permeabilized A549 cells for detection of TYK-2 using Anti-TYK-2 Rabbit Polyclonal Antibody (Product # 720124, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TYK2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cyt... View More

Product Image Gallery

TYK2 Antibody — Loss of Tyk2/TYK2 in JW23.3 murine MPNST and MPNST 724 cells leads to increased cell death. shTyk2-infected JW23.3 cell lines and control shLacZ: (A) Western blot densitometry analysis measuring TYK2 protein. (B) Relative Expression of Tyk2 mRNA compared to Gapdh. (C) An Incucyte cell proliferation assay measuring confluence over time. (D) An Incucyte death assay measuring TOTOTM-3 iodide fluorescence as an indicator of death over time. TYK2 knockout MPNST 724 cells and scramble control: (E) Western blot densitometry analysis of TYK2 protein. (F) Relative Expression of TYK2 mRNA compared to GAPDH. (G) An Incucyte cell proliferation assay measuring confluence over time. (H) An Incucyte death assay measuring TOTOTM-3 iodide fluorescence as an indicator of death over time. (*P < 0.05, **P < 0.01, ***P < 0.001) {KD}

产品信息

720124

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1 µg/mL

产品规格

种属反应

Human, Non-human primate

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human TYK2 (aa 439-646)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.1% BSA, 30% glycerol

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2609023

产品详细信息

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

靶标信息

Tyrosine Kinase 2 (TYK2) a member of the JAK kinase family, plays both structural and catalytic roles in type I interferon (IFN) signaling. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with hyperimmunoglobulin E syndrome (HIES) - a primary immunodeficiency characterized by elevated serum immunoglobulin E.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

TYK2 promotes malignant peripheral nerve sheath tumor progression through inhibition of cell death.

Cancer medicine

Qin W,Godec A,Zhang X,Zhu C,Shao J,Tao Y,Bu X,Hirbe AC

720124 was used in Western Blotting to illustrate the importance of TYK2 in MPNST pathogenesis and suggest that the TYK2 pathway may be a potential therapeutic target for these deadly cancers.