Invitrogen

USP9X Monoclonal Antibody (OTI2G7)

USP9X Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

USP9X Antibody (MA5-26367) in ICC/IF

Immunofluorescence analysis of Probable ubiquitin carboxyl-terminal hydrolase FAF-X was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with USP9X Monoclonal Antibody (OTI2G7) (Product # MA5-26367) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinan... View More

USP9X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

USP9X Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with Probable ubiquitin carboxyl-terminal hydrolase FAF-X siRNA and decrease in signal intensity was observed in Western Blot application using Anti-USP9X Monoclonal Antibody (OTI2G7) (Product # MA5-26367). {KD}

产品信息

MA5-26367

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:150

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

OTI2G7

抗原

Human recombinant protein fragment corresponding to amino acids 2246-2570 of USP9X produced in E.coli

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2723770

靶标信息

USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9x has a critical function in tight junction biogenesis by controlling the levels of the exchange factor for Arf6 (EFA6), a protein shown to facilitate tight junction formation during a narrow temporal window preceding the establishment of cell polarity. USP9X is also located on the X-chromosome but escapes X-inactivation, and defects in USP9X may cause gonadal degeneration in Turner syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Deubiquitinating enzyme FAF-X; Drosophila fat facets related, X-linked; Fat facets homolog; Fat facets in mammals; fat facets protein related, X-linked; Fat facets protein-related, X-linked; fats facets protein related, X; hFAM; probable ubiquitin carboxyl-terminal hydrolase FAF-X; Ubiquitin carboxyl-terminal hydrolase 9X; Ubiquitin carboxyl-terminal hydrolase FAM; ubiquitin specific peptidase 9, X chromosome; ubiquitin specific protease 9 SSSRF- isoform; ubiquitin specific protease 9, X chromosome; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); Ubiquitin thioesterase FAF-X; ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X

查看更多收起

基因别名: 5730589N07Rik; AA407302; AA407699; AL022658; AL022749; DFFRX; FAF; FAF-X; Fafl; FAM; MRX99; MRXS99F; RGD1560056; USP9; USP9X

查看更多收起

UniProt ID: (Human) Q93008, (Mouse) P70398

查看更多收起

Entrez Gene ID: (Dog) 480885, (Human) 8239, (Mouse) 22284, (Rat) 363445

查看更多收起

功能

cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity protein binding cysteine-type peptidase activity co-SMAD binding peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity cysteine protease

参与通路