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Invitrogen
WNT3A Recombinant Rabbit Monoclonal Antibody (1H12L14)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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WNT3A Antibody (703666) in ICC/IF
For immunofluorescence analysis, Hep G2 cells were fixed and permeabilized for detection of endogenous WNT3A using Anti-WNT3A Recombinant Rabbit Monoclonal Antibody (Product # 703666, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows represent... View More
产品信息
703666
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
1H12L14
抗原
Peptides corresponding to human WNT3A [1) aa168-aa185 2) aa262-aa280]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2784597
产品详细信息
This antibody is predicted to react with Monkey, Pig, Mouse, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC119418; MGC119419; MGC119420; Protein Wnt-3a; wingless-type MMTV integration site family member 3A; wingless-type MMTV integration site family, member 3A; Wnt 3a; WNT3A protein
基因别名: WNT3A
UniProt ID: (Human) P56704
Entrez Gene ID: (Human) 89780
osteoblast differentiation
in utero embryonic development
positive regulation of cytokine production
positive regulation of protein phosphorylation
heart looping
positive regulation of receptor internalization
negative regulation of heart induction by canonical Wnt signaling pathway
axon guidance
synapse assembly
positive regulation of cell proliferation
COP9 signalosome assembly
negative regulation of neuron projection development
Wnt signaling pathway
spinal cord association neuron differentiation
hippocampus development
cell proliferation in forebrain
Wnt signaling pathway involved in forebrain neuroblast division
dorsal/ventral neural tube patterning
hemopoiesis
neuron differentiation
extracellular matrix organization
mammary gland development
positive regulation of B cell proliferation
positive regulation of protein binding
positive regulation of peptidyl-serine phosphorylation
cell proliferation in midbrain
cellular protein localization
positive regulation of catenin import into nucleus
skeletal muscle cell differentiation
post-anal tail morphogenesis
synaptic vesicle recycling
inner ear morphogenesis
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of mesodermal cell fate specification
paraxial mesodermal cell fate commitment
positive regulation of skeletal muscle tissue development
positive regulation of collateral sprouting in absence of injury
negative regulation of axon extension involved in axon guidance
negative regulation of neurogenesis
positive regulation of sequence-specific DNA binding transcription factor activity
palate development
canonical Wnt signaling pathway
positive regulation of protein tyrosine kinase activity
positive regulation of dermatome development
canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment
regulation of microtubule cytoskeleton organization
platelet aggregation
cellular response to retinoic acid
axis elongation involved in somitogenesis
positive regulation of canonical Wnt signaling pathway
positive regulation of protein localization to plasma membrane
negative regulation of dopaminergic neuron differentiation
beta-catenin destruction complex disassembly
Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation
positive regulation of cell-cell adhesion mediated by cadherin
positive regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation
positive regulation of neural precursor cell proliferation
positive regulation of hepatocyte proliferation
positive regulation of transcription regulatory region DNA binding
positive regulation of cardiac muscle cell differentiation
