Invitrogen

beta Amyloid Recombinant Rabbit Monoclonal Antibody (H31L21)

beta Amyloid Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 1

beta Amyloid Antibody (700254) in IHC (P)

Immunohistochemistry analysis of beta-Amyloid in formalin-fixed, paraffin-embedded human brain (left) and transgenic mouse tissue that expresses FAD mutant APP and PS1 (right) using a beta-Amyloid monoclonal antibody (Product # 700254) at a dilution of 1 µg/mL. Results show strong cytoplasmic staining in amyloid plaque.

Product Image Gallery

产品信息

700254

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看16篇已发表文章 16篇已发表文章

免疫组化（石蜡） (IHC (P))

1 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

查看2篇已发表文章 2篇已发表文章

免疫组织化学（悬浮细胞） (IHC (Free))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看7篇已发表文章 7篇已发表文章

酶联免疫吸附实验 (ELISA)

0.1-0.2 µg/mL

查看2篇已发表文章 2篇已发表文章

Luminex (LUM)

查看1篇已发表文章 1篇已发表文章

点杂交 (DB)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Goat, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

H31L21

抗原

A peptide corresponding to amino acids 707-713 of P05067.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532306

产品详细信息

Cross reactivity to Abeta [1-40] is not observed in sandwich ELISA. In addition, in antigen ELISA cross-reactivity is not observed with Abeta [1-37], Abeta [1-38], Abeta [1-40], or Abeta [1-43] when used at low antibody concentrations (up to 30 ng/mL).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimer’s disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down’s syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Multimodal Assessment of Bottlenose Dolphin Auditory Nuclei Using 7-Tesla MRI, Immunohistochemistry and Stereology.

Veterinary sciences

Orekhova K,Selmanovic E,De Gasperi R,Gama Sosa MA,Wicinski B,Maloney B,Seifert A,Alipour A,Balchandani P,Gerussi T,Graïc JM,Centelleghe C,Di Guardo G,Mazzariol S,Hof PR

700254 was used in Immunohistochemistry to assess the connectivity of the inferior colliculi and ventral cochlear nuclei using diffusion tensor imaging (DTI).

Tue Dec 13 00:00:00 UTC 2022

Farnesyltransferase inhibitor LNK-754 attenuates axonal dystrophy and reduces amyloid pathology in mice.

Molecular neurodegeneration

Cuddy LK,Alia AO,Salvo MA,Chandra S,Grammatopoulos TN,Justman CJ,Lansbury PT,Mazzulli JR,Vassar R

700254 was used in Immunohistochemistry-immunofluorescence to show that the results agree with previous work identifying FTase as a therapeutic target for treating proteinopathies and could have important therapeutic implications in treating AD.

Sat Aug 20 00:00:00 UTC 2022

Amyloid Pathology in the Central Auditory Pathway of 5XFAD Mice Appears First in Auditory Cortex.

Journal of Alzheimer's disease : JAD

Weible AP,Wehr M

700254 was used in Immunohistochemistry to suggest that Aβ42 accumulation, but not plaques, may impair gap detection.

Tue Oct 18 00:00:00 UTC 2022

Oral nimodipine treatment has no effect on amyloid pathology or neuritic dystrophy in the 5XFAD mouse model of amyloidosis.

PloS one

Sadleir KR,Popovic J,Khatri A,Vassar R

700254 was used in Dot blot to conclude that nimodipine treatment is not likely to improve amyloid pathology or decrease neuritic dystrophy in AD, it is worth noting that nimodipine did not worsen the phenotype suggesting its use is safe in AD patients.

Fri Feb 25 00:00:00 UTC 2022

Methodology and Neuromarkers for Cetaceans' Brains.

Veterinary sciences

Sacchini S,Herráez P,Arbelo M,Espinosa de Los Monteros A,Sierra E,Rivero M,Bombardi C,Fernández A

700254 was used in Immunohistochemistry to propose a cetacean brain sampling protocol for neuroanatomical and neuropathological studies, including neurodegeneration, complemented by different neuromarkers useful for a complete diagnosis of the brain status.

Fri Jan 21 00:00:00 UTC 2022

Neuronal spreading and plaque induction of intracellular Aβ and its disruption of Aβ homeostasis.

Acta neuropathologica

Roos TT,Garcia MG,Martinsson I,Mabrouk R,Israelsson B,Deierborg T,Kobro-Flatmoen A,Tanila H,Gouras GK

700254 was used in Immunocytochemistry, Immunohistochemistry to indicate an essential role for intracellular prion-like Aβ and its synaptic spread in the pathogenesis of AD.

Fri Oct 01 00:00:00 UTC 2021

Neuronal spreading and plaque induction of intracellular Aβ and its disruption of Aβ homeostasis.

Acta neuropathologica

Roos TT,Garcia MG,Martinsson I,Mabrouk R,Israelsson B,Deierborg T,Kobro-Flatmoen A,Tanila H,Gouras GK

700254 was used in Immunocytochemistry, Immunohistochemistry to indicate an essential role for intracellular prion-like Aβ and its synaptic spread in the pathogenesis of AD.

Fri Oct 01 00:00:00 UTC 2021

Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype: Involvement of proteasome activation.

Free radical biology & medicine

Mladenovic Djordjevic AN,Kapetanou M,Loncarevic-Vasiljkovic N,Todorovic S,Athanasopoulou S,Jovic M,Prvulovic M,Taoufik E,Matsas R,Kanazir S,Gonos ES

700254 was used in Immunocytochemistry to test if the activation of proteasome in vivo can alleviate Alzheimer's disease pathology.

Fri Jan 01 00:00:00 UTC 2021

Metabolic, Phenotypic, and Neuropathological Characterization of the Tg4-42 Mouse Model for Alzheimer's Disease.

Journal of Alzheimer's disease : JAD

Hinteregger B,Loeffler T,Flunkert S,Neddens J,Bayer TA,Madl T,Hutter-Paier B

700254 was used in Immunohistochemistry to provide a comprehensive characterization of the Tg4-42 mouse model which could lead to a deeper understanding of pathological features of AD.

Tue Sep 14 00:00:00 UTC 2021

Extracellular Protein Aggregates Colocalization and Neuronal Dystrophy in Comorbid Alzheimer's and Creutzfeldt-Jakob Disease: A Micromorphological Pilot Study on 20 Brains.

International journal of molecular sciences

Jankovska N,Olejar T,Matej R

700254 was used in Immunohistochemistry (Paraffin) to show that PrPSc aggregation could dominate during co-aggregation with non-compact Aβ in the periphery of Aβ plaques.

Sat Feb 20 00:00:00 UTC 2021

Pregabalin Treatment does not Affect Amyloid Pathology in 5XFAD Mice.

Current Alzheimer research

Sadleir KR,Popovoic J,Zhu W,Reidel CT,Do H,Silverman RB,Vassar R

700254 was used in Dot Blot to test the hypothesis that increased resting calcium in neuronal processes near amyloid plaques plays a role in the development of neuritic dystrophies and further progression of amyloid pathology.

Thu Nov 18 00:00:00 UTC 2021

Identification of key transcriptome biomarkers based on a vital gene module associated with pathological changes in Alzheimer's disease.

Aging

Zhang T,Shen Y,Guo Y,Yao J

700254 was used in Western Blot to identify several key transcriptome biomarkers, which promoted the prediction and diagnosis of AD.

Mon May 24 00:00:00 UTC 2021

Extracellular Prion Protein Aggregates in Nine Gerstmann-Sträussler-Scheinker Syndrome Subjects with Mutation P102L: A Micromorphological Study and Comparison with Literature Data.

International journal of molecular sciences

Jankovska N,Matej R,Olejar T

700254 was used in Immunohistochemistry to study GSS plaques' micromorphology and protein composition. Found small, condensed plaques dominate; dystrophic neuritic changes with tau or Aβ rare and unrelated to GSS development.

Fri Dec 10 00:00:00 UTC 2021

Alzheimer's disease BIN1 coding variants increase intracellular Aβ levels by interfering with BACE1 recycling.

The Journal of biological chemistry

Perdigão C,Barata MA,Burrinha T,Guimas Almeida C

700254 was used in Immunocytochemistry to demonstrate that the LOAD-coding variants in Bin1 lead to a loss of function in endocytic recycling, which may be an early causal mechanism of LOAD.

Wed Sep 01 00:00:00 UTC 2021

Synergistic toxicity between tau and amyloid drives neuronal dysfunction and neurodegeneration in transgenic C. elegans.

Human molecular genetics

Benbow SJ,Strovas TJ,Darvas M,Saxton A,Kraemer BC

700254 was used in Luminex to generate a transgenic Caenorhabditis elegans model expressing both human Aβ1-42 peptide and human tau protein pan-neuronally to refine strategies for therapeutic discovery and development for Alzheimer's disease.

Sat Feb 01 00:00:00 UTC 2020

Comparison of regional flortaucipir PET with quantitative tau immunohistochemistry in three subjects with Alzheimer's disease pathology: a clinicopathological study.

EJNMMI research

Pontecorvo MJ,Keene CD,Beach TG,Montine TJ,Arora AK,Devous MD,Navitsky M,Kennedy I,Joshi AD,Lu M,Serrano GE,Sue LI,Intorcia AJ,Rose SE,Wilson A,Hellstern L,Coleman N,Flitter M,Aldea P,Fleisher AS,Mintun MA,Siderowf A

700254 was used in Immunohistochemistry to make a quantitative comparison of flortaucipir PET retention with pathological tau and β-amyloid across a range of brain regions at autopsy.

Mon Jun 15 00:00:00 UTC 2020

Microglial response to experimental periodontitis in a murine model of Alzheimer's disease.

Scientific reports

Kantarci A,Tognoni CM,Yaghmoor W,Marghalani A,Stephens D,Ahn JY,Carreras I,Dedeoglu A

700254 was used in Immunohistochemistry to suggest that microglia is central to the association between PD and AD.

Thu Oct 29 00:00:00 UTC 2020

TREM2 function impedes tau seeding in neuritic plaques.

Nature neuroscience

Leyns CEG,Gratuze M,Narasimhan S,Jain N,Koscal LJ,Jiang H,Manis M,Colonna M,Lee VMY,Ulrich JD,Holtzman DM

700254 was used in Immunohistochemistry to demonstrate a key role for triggering receptor expressed on myeloid cells 2 and microglia in limiting the development of peri-plaque tau pathologies.

Thu Aug 01 00:00:00 UTC 2019

Dual dose-dependent effects of fingolimod in a mouse model of Alzheimer's disease.

Scientific reports

Carreras I,Aytan N,Choi JK,Tognoni CM,Kowall NW,Jenkins BG,Dedeoglu A

700254 was used in Immunohistochemistry to study the effects of fingolimod, a structural analog of sphingosine, and suggests that, unlike the case in multiple sclerosis, fingolimod may potentially have therapeutic benefits in AD at low doses that do not affect peripheral lymphocyte function.

Mon Jul 29 00:00:00 UTC 2019

Alzheimer's Disease-Associated β-Amyloid Is Rapidly Seeded by Herpesviridae to Protect against Brain Infection.

Neuron

Eimer WA,Vijaya Kumar DK,Navalpur Shanmugam NK,Rodriguez AS,Mitchell T,Washicosky KJ,György B,Breakefield XO,Tanzi RE,Moir RD

700254 was used in Immunohistochemistry-Immunofluorescence to study the effects of herpesvirus surface glycoproteins on Alzheimer's disease-associated Β-amyloid deposition.

Wed Jul 11 00:00:00 UTC 2018

Chronic oral application of a periodontal pathogen results in brain inflammation, neurodegeneration and amyloid beta production in wild type mice.

PloS one

Ilievski V,Zuchowska PK,Green SJ,Toth PT,Ragozzino ME,Le K,Aljewari HW,O'Brien-Simpson NM,Reynolds EC,Watanabe K

700254 was used in Immunohistochemistry-immunofluorescence to study whether repeated exposure of wild type C57BL/6 mice to orally administered porphyromonas gingivalis results in pathognomonic signs of Alzheimer's disease.

Thu Mar 14 00:00:00 UTC 2019

ER stress is not elevated in the 5XFAD mouse model of Alzheimer's disease.

The Journal of biological chemistry

Sadleir KR,Popovic J,Vassar R

700254 was used in Dot blot to evaluate whether 5XFAD mice have increased endoplasmic reticulum stress.

Fri Nov 30 00:00:00 UTC 2018

Identification of natural products with neuronal and metabolic benefits through autophagy induction.

Autophagy

Fan Y,Wang N,Rocchi A,Zhang W,Vassar R,Zhou Y,He C

700254 was used in dot blot and immunohistochemistry to identify autophagy-inducing phytochemicals

Mon Jan 02 00:00:00 UTC 2017

Bin1 and CD2AP polarise the endocytic generation of beta-amyloid.

EMBO reports

Ubelmann F,Burrinha T,Salavessa L,Gomes R,Ferreira C,Moreno N,Guimas Almeida C

700254 was used in Immunofluorescence to identify a novel neuron-specific polarisation of beta-amyloid generation controlled by Bin1 and CD2AP.

Sun Jan 01 00:00:00 UTC 2017

Heterogeneous Association of Alzheimer's Disease-Linked Amyloid-β and Amyloid-β Protein Precursor with Synapses.

Journal of Alzheimer's disease : JAD

Willén K,Sroka A,Takahashi RH,Gouras GK

700254 was used in Immunocytochemistry-immunoflourescence to demonstrate that AΒ binds to a subset of synapses in cultured neurons, preferentialy to glutamatergic rather than GABAergic neurons.

Mon Apr 09 00:00:00 UTC 2018

The fornix provides multiple biomarkers to characterize circuit disruption in a mouse model of Alzheimer's disease.

NeuroImage

Badea A,Kane L,Anderson RJ,Qi Y,Foster M,Cofer GP,Medvitz N,Buckley AF,Badea AK,Wetsel WC,Colton CA

700254 was used in immunohistochemistry to propose that the fornix plays a role in Alzheimer's disease

Tue Nov 15 00:00:00 UTC 2016

Neutral Sphingomyelinase-2 Deficiency Ameliorates Alzheimer's Disease Pathology and Improves Cognition in the 5XFAD Mouse.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Dinkins MB,Enasko J,Hernandez C,Wang G,Kong J,Helwa I,Liu Y,Terry AV,Bieberich E

700254 was used in ELISA, immunohistochemistry, and western blot to report that reduced exosome secretion due to nSMase2 loss improves pathology and cognition in a mouse model of Alzheimer's disease

Wed Aug 17 00:00:00 UTC 2016

Early Alzheimer's disease-type pathology in the frontal cortex of wild mountain gorillas (Gorilla beringei beringei).

Neurobiology of aging

Perez SE,Sherwood CC,Cranfield MR,Erwin JM,Mudakikwa A,Hof PR,Mufson EJ

700254 was used in immunohistochemistry - free floating to characterize the frontal cortex of wild mountain gorillas (Gorilla beringei beringei) for early Alzheimer's disease-type pathology

Tue Mar 01 00:00:00 UTC 2016

Active duty service members who sustain a traumatic brain injury have chronically elevated peripheral concentrations of Aβ40 and lower ratios of Aβ42/40.

Brain injury

Lejbman N,Olivera A,Heinzelmann M,Feng R,Yun S,Kim HS,Gill J

700254 was used in ELISA to investigate if amyloid beta 40 or 42 is related to sustaining a traumatic brain injury or to chronic neurological symptoms in a young cohort of military personnel

Wed Jan 03 00:00:00 UTC 2018

Cerebral cortical Aβ42 and PHF-τ in 325 consecutive brain autopsies stratified by diagnosis, location, and APOE.

Journal of neuropathology and experimental neurology

Postupna N,Keene CD,Crane PK,Gonzalez-Cuyar LF,Sonnen JA,Hewitt J,Rice S,Howard K,Montine KS,Larson EB,Montine TJ

700254 was used in immunohistochemistry - paraffin section to quantify amyloid beta 42 and paired helical filament-tau in the frontal, temporal, and parietal cortices

Sun Feb 01 00:00:00 UTC 2015

Exosome reduction in vivo is associated with lower amyloid plaque load in the 5XFAD mouse model of Alzheimer's disease.

Neurobiology of aging

Dinkins MB,Dasgupta S,Wang G,Zhu G,Bieberich E

700254 was used in immunohistochemistry to study the role of exosome function in Abeta plaque formation and the therapeutic potential of inhibiting neutral sphingomyelinase-2

Fri Aug 01 00:00:00 UTC 2014

Tonic inhibition in dentate gyrus impairs long-term potentiation and memory in an Alzheimer's [corrected] disease model.

Nature communications

Wu Z,Guo Z,Gearing M,Chen G

700254 was used in immunocytochemistry to investigate the contribution of GABA inhibition in the dentate gyrus in Alzheimer's disease

Fri Jun 13 00:00:00 UTC 2014

Hypersensitivity of the hippocampal CA3 region to stress-induced neurodegeneration and amyloidogenesis in a rat model of surgical menopause.

Brain : a journal of neurology

Zhang QG,Wang RM,Scott E,Han D,Dong Y,Tu JY,Yang F,Reddy Sareddy G,Vadlamudi RK,Brann DW

700254 was used in immunohistochemistry to study the effects of surgical menopause on the sensitivity of the hippocampal CA3 region to ischemic stress, amyloidogenesis and Abeta42 toxicity

Wed May 01 00:00:00 UTC 2013

Overexpression of Hsp27 ameliorates symptoms of Alzheimer's disease in APP/PS1 mice.

Cell stress & chaperones

Tóth ME,Szegedi V,Varga E,Juhász G,Horváth J,Borbély E,Csibrány B,Alföldi R,Lénárt N,Penke B,Sántha M

700254 was used in immunohistochemistry to study the beneficial effects of HSP27 overexpression in a murine model of Alzheimer's disease

Fri Nov 01 00:00:00 UTC 2013

Alzheimer's disease pathology in the neocortex and hippocampus of the western lowland gorilla (Gorilla gorilla gorilla).

The Journal of comparative neurology

Perez SE,Raghanti MA,Hof PR,Kramer L,Ikonomovic MD,Lacor PN,Erwin JM,Sherwood CC,Mufson EJ

700254 was used in immunohistochemistry to study the pathology of Alzheimer's disease in the western lowland Gorilla

Sun Dec 15 00:00:00 UTC 2013

Hypoxia due to cardiac arrest induces a time-dependent increase in serum amyloid β levels in humans.

PloS one

Zetterberg H,Mörtberg E,Song L,Chang L,Provuncher GK,Patel PP,Ferrell E,Fournier DR,Kan CW,Campbell TG,Meyer R,Rivnak AJ,Pink BA,Minnehan KA,Piech T,Rissin DM,Duffy DC,Rubertsson S,Wilson DH,Blennow K

700254 was used in ELISA to study the effects of hypoxia following cardiac arrest on Abeta42 serum levels in humans

Mon Aug 27 00:00:00 UTC 2012

生物信息学

蛋白别名: ABPP; AG; Alzheimer disease; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; amyloid beta A4 protein; Amyloid precursor protein; Amyloid β; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; Amyloidogenic glycoprotein; APP; APPI; appican; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CTF gamma; CTF-alpha; CVAP; OTTHUMP00000096096; Pan-Abeta; peptidase nexin-II; PN-II; PreA4; PreA4 751; protease nexin II; Protease nexin-II; testicular tissue protein Li 2

查看更多收起

基因别名: A4; AAA; ABETA; ABPP; AD1; Adap; Ag; APP; APPI; betaApp; CTFgamma; CVAP; E030013M08Rik; PN-II; PN2

查看更多收起

UniProt ID: (Human) P05067, (Mouse) P12023

查看更多收起

Entrez Gene ID: (Human) 351, (Mouse) 11820

查看更多收起

功能