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Invitrogen
| 应用 | 建议稀释比 |
|---|---|
免疫细胞化学 (ICC/IF) |
1:500-1:20,000 |
流式细胞分析 (Flow) |
1:500-1:20,000 |
酶联免疫吸附实验 (ELISA) |
1:500-1:20,000 |
| 产品规格 | |
|---|---|
种属反应 |
Human |
宿主/亚型 |
Goat / IgG |
分类 |
Polyclonal |
类型 |
Secondary Antibody |
抗原 |
Human IgG/A/M |
偶联物 |
Biotin
|
形式 |
Liquid |
纯化类型 |
purified |
保存液 |
PBS, pH 7.2, with 1% BSA |
内含物 |
0.05% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2535967 |
靶标 |
IgG/IgA/IgM |
抗体形式 |
F(ab')2 Fragment |
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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