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| 应用 | 建议稀释比 |
|---|---|
免疫印迹 (WB) |
Assay-dependent |
免疫组化 (IHC) |
Assay-dependent |
免疫细胞化学 (ICC/IF) |
Assay-dependent |
原位杂交 (ISH) |
Assay-dependent |
| 产品规格 | |
|---|---|
种属反应 |
Human |
宿主/亚型 |
Goat / IgG |
分类 |
Polyclonal |
类型 |
Secondary Antibody |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
浓度 |
1 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.8 |
内含物 |
0.08% sodium azide |
保存条件 |
4° C |
RRID |
AB_228277 |
靶标 |
IgM |
抗体形式 |
Whole Antibody |
Product # 31124 has been successfully used in Western blot, IF, ICC, IHC and ISH applications.
The activity measured by a solid phase binding assay is 91%.
Product # 31124 reacts with the µ chains of human IgM. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with dog IgM (12%) and mouse IgM (12%). It has minimum cross-reactivity with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 0.5 mL of distilled water (1.0 mg/mL after restoration).
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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