Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
| 应用 | 建议稀释比 |
|---|---|
免疫印迹 (WB) |
1:500-1:20,000 |
免疫组化 (IHC) |
1:500-1:20,000 |
酶联免疫吸附实验 (ELISA) |
1:500-1:20,000 |
| 产品规格 | |
|---|---|
种属反应 |
Mouse |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Secondary Antibody |
抗原 |
Gamma Immunoglobins Heavy and Light chains |
偶联物 |
AP
|
形式 |
Liquid |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2, with 30mM triethanolamine, 1% BSA |
内含物 |
0.05% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2534834 |
靶标 |
IgG |
抗体形式 |
Whole Antibody |
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
