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Invitrogen
| 应用 | 建议稀释比 |
|---|---|
免疫细胞化学 (ICC/IF) |
0.1-1 µg/mL |
| 产品规格 | |
|---|---|
种属反应 |
Mouse |
宿主/亚型 |
Rabbit / IgG |
分类 |
Recombinant Polyclonal |
类型 |
Secondary Antibody |
抗原 |
Recombinant full-length protein |
偶联物 |
Alexa Fluor™ 594
|
激发/发射光谱 |
查看光谱
|
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
Gravity column chromatography |
保存液 |
PBS |
内含物 |
5mM sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2536090 |
靶标 |
IgG |
抗体形式 |
Whole Antibody |
The sensitivity and specificity of each lot is confirmed using ELISA.
Minimal cross-reactivity with goat, rat, human, bovine, guinea pig, and donkey IgG is observed.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
