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| 应用 | 建议稀释比 |
|---|---|
免疫印迹 (WB) |
1:2,000-1:10,000 |
免疫组化 (IHC) |
1:1,000-1:5,000 |
酶联免疫吸附实验 (ELISA) |
1:20,000-1:100,000 |
| 产品规格 | |
|---|---|
种属反应 |
Rat |
宿主/亚型 |
Sheep / IgG |
分类 |
Polyclonal |
类型 |
Secondary Antibody |
抗原 |
Rat IgG whole molecule |
偶联物 |
Biotin
|
形式 |
Liquid |
浓度 |
2 mg/mL |
纯化类型 |
IgG fraction |
保存液 |
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA |
内含物 |
0.01% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_11006232 |
靶标 |
IgG |
抗体形式 |
Whole Antibody |
PA1-28641 detects Rat IgG from rat samples.
Thermo Scientific Pierce Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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